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Expression profile analysis of the polygalacturonase-inhibiting protein genes in rice and their responses to phytohormones and fungal infection

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Abstract

Polygalacturonase-inhibiting proteins (PGIPs) are typically leucine-rich repeat (LRR) proteins that can inhibit the activity of fungal polygalacturonases (PGs). In this study, two new Ospgip genes, named Ospgip6 and Ospgip7 with consensus sequence of ten imperfect LRR motif located on rice chromosomes 8 and 9, were identified using BLAST analysis. Both of them appear to be extracellular glycoproteins. To have a global view of the dynamic gene expression pattern, seven Ospgip genes were first analyzed using the Affymetrix rice genome array data from online resource. All of these seven Ospgip genes showed variable expression patterns among tissues/organs. In order to further investigate the potential function of these Ospgip genes, the responses of Ospgip genes to the treatment of various phytohormones (abscisic acid, brassinosteroid, gibberellic acid, 3-indole acetic acid, jasmonic acid, kinetin, naphthalene acetic acid and salicylic acid) as well as fungal infection were analyzed by real-time PCR using time course array. Generally, all the Ospgip genes were slightly up-regulated in the indica rice cultivar Minghui 63 under GA3, KT and NAA treatments (except Ospgip2, which was down-regulated under KT treatment). In the japonica rice cultivar Zhonghua 11, Ospgip genes were regulated by most treatments with the response time variability. We also analyzed putative cis-elements in the promoter regions of Ospgip genes. This dataset provided a versatile resource to understand the regulatory network of Ospgip genes during the process of phytohormones treatment and fungal infection in the model monocotyledonous plant, rice, and could aid in the transgenic breeding against rice fungal diseases.

Key message

All the seven Ospgip genes showed variable expression patterns in Minghui 63 and their expressions were regulated by different phytohormone treatments or fungal infection in Minghui 63 and Zhonghua 11.

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Abbreviations

ABA:

Abscisic acid

BR:

Brassinosteroid

GA3 :

Gibberellic acid

IAA:

3-Indole acetic acid

JA:

Jasmonic acid

KT:

Kinetin

NAA:

Naphthalene acetic acid

SA:

Salicylic acid

LRR:

Leucine-rich repeat

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Acknowledgments

This research was funded by the National Natural Science Foundation of China, the National High Technology Research and Development Program of China (863 Program) and the National Program on Research and Development of Transgenic Plants. We would like to thank Dr. M.W. Szczerba for helpful discussion and comments.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Liaoxun Lu, Fei Zhou, Yong Zhou, Xiaolei Fan, Lei Wang, Hao Chen & Yongjun Lin

  2. Shanghai Agrobiological Gene Center, Shanghai, 201106, People’s Republic of China

    Shuifeng Ye

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  1. Liaoxun Lu
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  2. Fei Zhou
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  3. Yong Zhou
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  4. Xiaolei Fan
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  7. Hao Chen
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Corresponding author

Correspondence to Yongjun Lin.

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Communicated by Y. Lu.

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Appendix: accession numbers

Appendix: accession numbers

Protein accession numbers

AdPGIP (Actinidia deliciosa, NCBI ID: CAA88846); AtPGIP1-2 (Arabidopsis thaliana, NCBI ID: AAF69827, AAF69828); BnPGIP1-3, 5-17 (Brassica napus, NCBI ID: ABX46548-ABX46563); CmPGIP (Chamaebatiaria millefolium, NCBI ID: AAK43398); CsPGIP (Citrus sinensis, NCBI ID: CAA69910); EgPGIP (Eucalyptus grandis, NCBI ID: AAF22248); GmPGIP1-4 (Glycine max, NCBI ID: CAI99392–CAI99395); HaPGIP (Helianthus annuus, NCBI ID: ABW89508); OsPGIP1-7(Oryza sativa, NCBI ID: CAJ55691, CAJ55692, CAJ55693, CAJ55694, AAO17320, NP_001062185, EEE69955); PaPGIP (Phaseolus acutifolius, NCBI ID: CAR92533); PcPGIP (Pyrus communis, NCBI ID: AAA33865); PpPGIP (Pyrus pyrifolia, NCBI ID: ACY56891); PvPGIP1-4 (Phaseolus vulgaris, NCBI ID: CAH10215–CAH10218); RsPGIP (Rhodotypos scandens, NCBI ID: AAK43455); SbPGIP1-2 (Sorghum bicolor, NCBI ID: XP_002463048, XP_002439097); SlPGIP (Solanum lycopersicum, NCBI ID: AAA53547); SpPGIP (Solanum palustre, NCBI ID: AAT77428); TaPGIP1-2 (Triticum aestivum, NCBI ID: CAJ55695, CAJ55696); VvPGIP (Vitis vinifera, NCBI ID: AAM74142); ZmPGIP1-2 (Zea mays, NCBI ID: NP_001147517, NP_001150670).

Nucleotide accession numbers

Atpgip1-2 (Arabidopsis thaliana, NCBI ID: AF229249, AF229250); Bnpgip1-3, 5-17 (Brassica napus, NCBI ID: EU142023-EU142038); Hapgip (Helianthus annuus, NCBI ID: EU112834); Ospgip1-6 (Oryza sativa, NCBI ID: AM180652–AM180655, NM_001066567, NM_001068720); Rspgip (Rhodotypos scandens, NCBI ID: AF196946); Sbpgip1-2 (Sorghum bicolor, NCBI ID: XM_002463003, NM_001157198); Zmpgip1-2 (Zea mays, NCBI ID: NM_001154045, NM_001157198).

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Lu, L., Zhou, F., Zhou, Y. et al. Expression profile analysis of the polygalacturonase-inhibiting protein genes in rice and their responses to phytohormones and fungal infection. Plant Cell Rep 31, 1173–1187 (2012). https://doi.org/10.1007/s00299-012-1239-7

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