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Identification of quantitative trait loci controlling rice mature seed culturability using chromosomal segment substitution lines

  • Genetics and Genomics
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Abstract

The genetic transformation efficiency of a rice variety is largely determined by its tissue culturability. Establishment of a highly efficient tissue-culture system has greatly accelerated the wide spread application of transgenic japonica varieties. However, such process for indica rice was hampered because this type of variety is recalcitrant to in vitro culture. This study aimed to map the quantitative trait loci (QTLs) for mature seed culturability using a chromosomal segment substitution lines (CSSL) population derived from a cross between an indica variety “Zhenshan 97B” and a japonica variety “Nipponbare”. The CSSLs consist of 139 lines each containing a single or a few introgression segments, and together covering the whole “Nipponbare” genome. Every CSSL was tested by culturing on the two medium systems developed for the respective indica and japonica parental varieties. The performance of culturability was evaluated by four indices: frequency of callus induction (CIF), callus subculture capability (CSC), frequency of plant regeneration (PRF) and the mean plantlet number per regenerated callus (MNR). All four traits displayed continuous variation among the CSSLs. With the culture system for japonica rice, three CIF QTLs, three CSC QTLs, three PRF QTLs and three MNR QTLs were detected. With the culture system for indica variety, six CIF QTLs, two CSC QTLs, three PRF QTLs and six MNR QTLs were identified, and these QTLs distributed on nine rice chromosomes. Two QTLs of CIF and two QTLs of MNR were detected in both the japonica and indica rice culture system. The correlation coefficients of all the four traits varied depending on the culture systems. These results provide the possibilities of enhancing the culturability of indica rice by marker-assisted breeding with those desirable alleles from the japonica.

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References

  • Agache S, De Buyser J, Henry Y, Snape JW (1988) Studies of the genetic relationship between anther culture and somatic tissue culture abilities in wheat. Plant Breed 100:26–33

    Article  Google Scholar 

  • Aldemita RR, Hodges TK (1996) Agrobacterium tumefaciens-mediated transformation of japonica and indica rice varieties. Planta 199:612–617

    Article  CAS  Google Scholar 

  • Amer IMB, Worland AJ, Korzun V, Borner A (1997) Genetic mapping of QTL controlling tissue-culture response on chromosome 2B of wheat (Triticum aestivum L.) in relation to major genes and RFLP markers. Theor Appl Genet 94:1047–1052

    Article  Google Scholar 

  • Armstrong CL, Romero-Severson J, Hodges TK (1992) Improved tissue culture response of an elite maize inbred through backcross breeding, and identification of chromosomal regions important for regeneration by RFLP analysis. Theor Appl Genet 84:755–762

    Article  Google Scholar 

  • Ashikari M, Matsuoka M (2006) Identification, isolation and pyramiding of quantitative trait loci for rice breeding. Trends Plant Sci 11(7):344–350

    Article  PubMed  CAS  Google Scholar 

  • Bingham ET, Hurley LV, Kaatz DM, Saunders JW (1975) Breeding alfalfa which regenerates from callus tissue in culture. Crop Sci 15:721

    Google Scholar 

  • Bolibok H, Rakoczy-Trojanowska M (2006) Genetic mapping of QTLs for tissue-culture response in plants. Euphytica 149:73–83

    Article  CAS  Google Scholar 

  • Chen QQ, Mu JX, Zhou HJ, Yu SB (2007) Genetic effect of japonica alleles detected in indica candidate introgression lines. Sci Agric Sin 40:2387–2394

    CAS  Google Scholar 

  • Chu CC, Wang CC, Sung CS, Hsu C, Yin CK, Chu CY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sin 18:659–668

    Google Scholar 

  • Churchill GA, Doerge RW (1994) Empirical threshold values for quantitative trait mapping. Genetics 138:963–971

    PubMed  CAS  Google Scholar 

  • Garg AK, Kim JK, Owens TG, Ranwala AP, Choi YD, Kochian LV, Wu RJ (2002) Trehalose accumulation in rice plants confers high tolerance levels to different abiotic stresses. Proc Natl Acad Sci USA 99:15898–15903

    Article  PubMed  CAS  Google Scholar 

  • Garin E, Bernier-Cardou M, Isabel N, Klimaszewska K, Plourde A (2000) Effect of sugars, amino acids, and culture technique on maturation of somatic embryos of Pinus strobus on medium with two gellan gum concentrations. Plant Cell Tissue Organ Cult 62:27–37

    Article  CAS  Google Scholar 

  • Harushima Y, Kurata N, Yano M, Nagamura Y, Sasaki T, Minobe Y, Nakagahra M (1996) Detection of segregation distortions in an indica-japonica rice cross using a high-resolution molecular map. Theor Appl Genet 92:145–150

    Article  CAS  Google Scholar 

  • He P, Shen L, Lu C, Chen Y, Zhu L (1998) Analysis of quantitative trait loci which contribute to anther culturability in rice (Oryza sativa L.). Mol Breed 4:165–172

    Article  CAS  Google Scholar 

  • Hiei Y, Obta S, Komari T, Kumashiro T (1994) Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of boundaries of T-DNA. Plant J 6:271–282

    Article  PubMed  CAS  Google Scholar 

  • Khanna HK, Raina SK (1999) Agrobacterium-mediated transformation of indica rice cultivars using binary and superbinary vectors. Aust J Plant Physiol 26:311–324

    Article  CAS  Google Scholar 

  • Komatsuda T, Taguchi-Shiobara F, Oka S, Takaiwa F, Annaka T, Jacobsen HJ (1995) Transfer and mapping of the shoot-differentiation locus Shd1 in barley chromosome 2. Genome 38:1009–1014

    Article  PubMed  CAS  Google Scholar 

  • Koornneef M, Bade J, Hanhart C, Horsman K, Schel J, Soppe W, Verkerk R, Zabel P (1993) Characterization and mapping of a gene controlling shoot regeneration in tomato. Plant J 3:131–141

    Article  CAS  Google Scholar 

  • Lazar MD, Chen THH, Scoles GJ, Kartha KK (1987) Immature embryo and anther culture of chromosome additions lines of rye in Chinese Spring wheat. Plant Sci 51:77–81

    Article  Google Scholar 

  • Lee K, Jeon H, Kim M (2002) Optimization of a mature embryo-based in vitro culture system for high-frequency somatic embryogenic callus induction and plant regeneration from japonica rice cultivars. Plant Cell Tissue Organ Cult 71:237–244

    Article  CAS  Google Scholar 

  • Li HH, Ye GY, Wang JK (2007) A modified algorithm for the improvement of composite interval mapping. Genetics 175:361–374

    Article  PubMed  Google Scholar 

  • Lin YJ, Zhang Q (2005) Optimising the tissue culture conditions for high efficiency transformation of indica rice. Plant Cell Rep 23:540–547

    Article  PubMed  CAS  Google Scholar 

  • Manninen OM (2000) Associations between anther-culture response and molecular markers on chromosomes 2H, 3H and 4H of barley (Hordeum vulgare L.). Theor Appl Genet 100:57–62

    Article  CAS  Google Scholar 

  • Mano Y, Komatsuda T (2002) Identification of QTLs controlling tissue-culture traits in barley (Hordeum vulgare L.). Theor Appl Genet 105:708–715

    Article  PubMed  CAS  Google Scholar 

  • Mohanty A, Kathuria H, Ferjani A, Sakamoto A, Mohanty P, Murata N, Tyagi AK (2002) Transgenics of an elite indica rice variety Pusa Basmati 1 harbouring the codA gene are highly tolerant to salt stress. Theor Appl Genet 106:51–57

    PubMed  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco cell cultures. Plant Physiol 15:473–497

    Article  CAS  Google Scholar 

  • Murigneux A, Bentolila S, Hardy T, Baud S, Guitton C, Jullien H, Ben Tahar S, Freyssinet G, Beckert M (1994) Genotypic variation of quantitative trait loci controlling in vitro androgenesis in maize. Genome 37:970–976

    Article  PubMed  CAS  Google Scholar 

  • Nishimura A, Ashikari M, Lin S, Takashi T, Angeles ER, Yamamoto T, Matsuoka M (2005) Isolation of a rice regeneration quantitative trait loci gene and its application to transformation systems. Proc Natl Acad Sci USA 102:11940–11944

    Article  PubMed  CAS  Google Scholar 

  • Peng J, Hodges TK (1989) Genetic analysis of plant regeneration in rice (Oryza sativa L.). In Vitro Cell Dev Biol 25:91–94

    Article  Google Scholar 

  • Taguchi-Shiobara F, Lin SY, Tanno K, Komatsuda T, Yano M, Sasaki T, Oka S (1997) Mapping quantitative trait loci associated with regeneration ability of seed callus in rice, Oryza sativa L. Theor Appl Genet 95:828–833

    Article  CAS  Google Scholar 

  • Taguchi-Shiobara F, Yamamoto T, Yano M, Oka S (2006) Mapping QTLs that control the performance of rice tissue culture and evaluation of derived near-isogenic lines. Theor Appl Genet 112:968–976

    Article  PubMed  CAS  Google Scholar 

  • Takeuchi Y, Abe T, Sasahara T (2000) RFLP mapping of QTLs influencing shoot regeneration from mature seed-derived calli in rice. Crop Sci 40:245

    CAS  Google Scholar 

  • Taylor TE, Veilleux RE (1992) Inheritance of competencies for leaf disc regeneration, anther culture, and protoplast culture in Solanum phureja and correlations among them. Plant Cell Tissue Organ Cult 31:95–103

    Article  Google Scholar 

  • Templeton-Sommers KM, Collins WW (1986) Heritability of regeneration in tissue cultures of sweet potato (Ipomoea batatas L.). Theor Appl Genet 71:836–841

    Article  Google Scholar 

  • Toki S, Hara N, Ono K, Onodera H, Tagiri A, Oka S, Tanaka H (2006) Early infection of scutellum tissue with Agrobacterium allows high-speed transformation of rice. Plant J 47:969–976

    Article  PubMed  CAS  Google Scholar 

  • Tsukahara M, Hirosawa T, Nagai E, Kato H, Ikeda R, Maruyama K (1995) Genetic analysis of plant regeneration ability in cell suspension cultures of rice (Oryza sativa L.). Breed Sci 45:425–428

    Google Scholar 

  • Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78

    Article  PubMed  CAS  Google Scholar 

  • Wan Y, Rocheford TR, Widholm JM (1992) RFLP analysis to identify putative chromosomal regions involved in the anther culture response and callus formation of maize. Theor Appl Genet 85:360–365

    Article  CAS  Google Scholar 

  • Wang JK, Wan XY, Li HH, Pfeiffer WH, Crouch J, Wan JM (2007) Application of identified QTL-marker associations in rice quality improvement through a design-breeding approach. Theor Appl Genet 115:87–100

    Article  PubMed  Google Scholar 

  • Zhang J, Xu RJ, Elliott MC, Chen DF (1997) Agrobacterium-mediated transformation of elite indica and japonica rice cultivars. Mol Biotechnol 8:223–231

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This research was funded by the National High Technology Research and Development Program of China (863 program) and the National Natural Science Foundation of China.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Lina Zhao, Hongju Zhou, Liaoxun Lu, Li Liu, Xianghua Li, Yongjun Lin & Sibin Yu

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  1. Lina Zhao
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  2. Hongju Zhou
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  3. Liaoxun Lu
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  4. Li Liu
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  5. Xianghua Li
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  6. Yongjun Lin
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  7. Sibin Yu
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Corresponding authors

Correspondence to Yongjun Lin or Sibin Yu.

Additional information

Communicated by K. Toriyama.

Lina Zhao and Hongju Zhou have contributed equally to this work.

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Zhao, L., Zhou, H., Lu, L. et al. Identification of quantitative trait loci controlling rice mature seed culturability using chromosomal segment substitution lines. Plant Cell Rep 28, 247–256 (2009). https://doi.org/10.1007/s00299-008-0641-7

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  • DOI: https://doi.org/10.1007/s00299-008-0641-7

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