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Towards normalization of soybean somatic embryo maturation

  • Cell Biology and Morphogenesis
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Abstract

Soybean (Glycine max L. Merrill) somatic embryos have been useful for assaying seed-specific traits prior to plant recovery. Such traits could be assessed more accurately if somatic embryos more closely mimicked seed development. Amino acid supplements, carbon source, and abscisic acid and basal salt formulations were tested in an effort to modify existing soybean embryogenesis histodifferentiation/maturation media to further normalize the development of soybean somatic embryos. The resultant liquid medium, referred to as soybean histodifferentiation and maturation medium (SHaM), consists of FNL basal salts, 3% sucrose, 3% sorbitol, filter-sterilized 30 mM glutamine and 1 mM methionine. SHaM-derived somatic embryos are more similar to seed in terms of protein and fatty acid/lipid composition, and conversion ability, than somatic embryos obtained from traditional soybean histodifferentiation and maturation media.

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Acknowledgements

This work was funded by a gift from Pioneer HiBred, International, a grant from the United Soybean Board, and from state and federal monies allocated to the Georgia Agricultural Experiment Stations

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Author notes

  1. M. A. Schmidt

    Present address: USDA/ARS at The Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, USA

Authors and Affiliations

  1. Center for Applied Genetic Technologies, 111 Riverbend Road, University of Georgia, Athens, GA 30602-6810, USA

    M. A. Schmidt, D. M. Tucker & W. A. Parrott

  2. USDA/ARS at The Donald Danforth Plant Science Center, 975 North Warson Road, St. Louis, MO 63132, USA

    E. B. Cahoon

Authors
  1. M. A. Schmidt
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  2. D. M. Tucker
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  3. E. B. Cahoon
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  4. W. A. Parrott
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Corresponding author

Correspondence to W. A. Parrott.

Additional information

Communicated by P. Ozias-Akins

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Schmidt, M.A., Tucker, D.M., Cahoon, E.B. et al. Towards normalization of soybean somatic embryo maturation. Plant Cell Rep 24, 383–391 (2005). https://doi.org/10.1007/s00299-005-0950-z

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  • DOI: https://doi.org/10.1007/s00299-005-0950-z

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