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Analysis of mycorrhizal associations formed by Cistus incanus transformed root clones with Terfezia boudieri isolates

  • Physiology and Biochemistry
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Abstract

One clone (M-2), out of several Agrobacterium rhizogenes transformed root clones of Cistus incanus, formed ecto- or endomycorrhiza in vitro with two isolates of Terfezia boudieri collected in Israel. All other clone-fungal isolate combinations formed ectomycorrhiza. The endomycorrhiza-forming isolate secreted smaller amounts of auxin than an ectomycorrhiza-forming isolate. Addition of 2,4-dichlorophenoxyacetic acid (2,4-D) led to ectomycorrhiza formation by the M-2 clone on low P medium. Endomycorrhizas were formed by both M-2 and a control clone with the same T. boudieri isolates on high P medium with 2,4-D. The M-2 clone of C. incanus exhibited greater sensitivity to exogenous auxins (IAA and 2,4-D) than other clones, and clonal sensitivity to auxin was increased tenfold under low P conditions. Results are discussed in relation to phosphate and auxin influence on T. boudieri–C. incanus interaction.

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Acknowledgment

We thank Dr. Zeev Wiesman for his advice and help in IAA analyses

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Authors and Affiliations

  1. Department of Life Sciences, Ben Gurion University, POB 653,, Beer Sheva, Israel

    M. Zaretsky & N. Roth-Bejerano

  2. The Institutes for Applied Research, Ben Gurion University, POB 653,, Beer Sheva, Israel

    V. Kagan-Zur & D. Mills

Authors
  1. M. Zaretsky
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  2. V. Kagan-Zur
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  3. D. Mills
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  4. N. Roth-Bejerano
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Corresponding author

Correspondence to N. Roth-Bejerano.

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Communicated by K. K. Kamo

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Zaretsky, M., Kagan-Zur, V., Mills, D. et al. Analysis of mycorrhizal associations formed by Cistus incanus transformed root clones with Terfezia boudieri isolates. Plant Cell Rep 25, 62–70 (2006). https://doi.org/10.1007/s00299-005-0035-z

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  • DOI: https://doi.org/10.1007/s00299-005-0035-z

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