The bacterial replisome has factory-like localization

  • Sarah M. Mangiameli
  • Julie A. Cass
  • Houra Merrikh
  • Paul A. Wiggins


DNA replication is essential to cellular proliferation. The cellular-scale organization of the replication machinery (replisome) and the replicating chromosome has remained controversial. Two competing models describe the replication process: In the track model, the replisomes translocate along the DNA like a train on a track. Alternately, in the factory model, the replisomes form a stationary complex through which the DNA is pulled. We summarize the evidence for each model and discuss a number of confounding aspects that complicate interpretation of the observations. We advocate a factory-like model for bacterial replication where the replisomes form a relatively stationary and weakly associated complex that can transiently separate.


DNA replication Replication factory Cell biology Bacteria 



The authors are grateful to Colin LaMont for the helpful comments on the manuscript.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Sarah M. Mangiameli
    • 1
  • Julie A. Cass
    • 2
  • Houra Merrikh
    • 3
  • Paul A. Wiggins
    • 1
  1. 1.Department of PhysicsUniversity of WashingtonSeattleUSA
  2. 2.Department of BiologyUniversity of WashingtonSeattleUSA
  3. 3.Department of MicrobiologyHealth Sciences BuildingSeattleUSA

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