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Chelation of zinc(II) with poly(γ-glutamic acid) in aqueous solution: kinetics, binding constant, and its antimicrobial activity

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Abstract

The binding of zinc(II) ions onto γ-pga was studied in aqueous solution as a function of contact time, solution pH, initial concentration of metal ion, and solution temperature, respectively. IR and 13C NMR spectra of γ-pga and Zn(γ-pga) complex revealed binding of zinc(II) with carboxylate and amide groups on γ-pga. The strong interaction between zinc(II) ions and γ-pga occurred at pH 5. The binding kinetic data followed a pseudo-second-order kinetic model. The equilibrium binding data were fitted well by Langmuir isotherm model. The maximum amount of zinc(II) ions bound to γ-pga was estimated to be 13.97 mmol/g at 30 °C and 20.58 mmol/g at 45 °C. The values of activation energy (Ea 18.23 kJ/mol), changes in free energy (∆G°), enthalpy (∆H°), and entropy (∆S°) indicate that the formation of Zn(γ-pga) complex is an endothermic spontaneous physisorption process. The antimicrobial activity of Zn(γ-pga) complex was examined against a gram-negative bacteria (i.e., Escherichia coli) and a gram-positive bacteria (i.e., Bacillus subtilis). The IC50 values of Zn(γ-pga) complex were estimated to be (0.76 ± 0.03) mmol/L for E. coli and (0.94 ± 0.02) mmol/L for B. subtilis, respectively. Therefore, Zn(γ-pga) complex can be used as an antimicrobial agent against gram-positive and gram-negative microorganisms.

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Acknowledgements

We gratefully acknowledge the support given to Prof. Dr. Tapan Kumar Saha by the University Grants Commission of Bangladesh to carry out this work (FY 2017-2018). We are thankful to Mr. Nikhil Chandra Bhoumik (Wazed Miah Science Research Center, Jahangirnagar University) for assisting in the use the atomic absorption spectroscopy (AAS) at the center.

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Authors and Affiliations

  1. Department of Chemistry, Jahangirnagar University, Savar, Dhaka, 1342, Bangladesh

    Bakul Akter, Ariful Islam Khan, Subarna Karmaker, Pollob Ghosh, Soma Saha & Tapan Kumar Saha

  2. Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka, 1342, Bangladesh

    Shakil Ahmed Polash, Zinia Islam, Satya Ranjan Sarker & Md. Sharif Hossain

  3. Division of Analytical and Physical Sciences, Department of Analytical and Bioinorganic Chemistry, Kyoto Pharmaceutical University, 5 Nakauchi-cho, Misasagi, Kyoto, 607-8414, Japan

    Hiroyuki Yasui

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  1. Bakul Akter
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Correspondence to Tapan Kumar Saha.

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Akter, B., Khan, A.I., Karmaker, S. et al. Chelation of zinc(II) with poly(γ-glutamic acid) in aqueous solution: kinetics, binding constant, and its antimicrobial activity. Polym. Bull. 78, 1353–1377 (2021). https://doi.org/10.1007/s00289-020-03165-9

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