This study aims the improvement of antibacterial and biocompatibility properties of electrospray ternary blends of chitosan/poly(ethylene glycol)/hyaluronic acid. It conserves microscale particle structure even after incorporating zinc oxide (ZnO), the zeolite Mobil Composition of Matter No. 41 (MCM41) and penicillin G during this technique. Three different electrospray (ESP) blend compositions (ESPI, ESPII and ESPIII) have been produced in order to improve both antibacterial activity against to both gram-positive and gram-negative bacteria and biocompatibility. Results of FTIR spectroscopy and microscopy verified with SEM, EDS and AFM analyses. Hyaluronic acid surface has been specified definitely through ZnO-based ESPI surface composed of heterogeneously dispersed microparticles. Surface structures of ESPII and ESPIII have more homogenously dispersed microparticles as hill–valley surface by the aid of MCM 41-PEN. Antibacterial activity has been performed by Kirby–Bauer method. ESPI has good antibacterial activity against both gram-positive (S. aureus and S. epidermidis) and gram-negative bacteria (E. cloacea). Each electrospray film displayed good biocompatibility against to mouse fibroblast cell line L929 (ATTC number CCL-1). The highest amount of cell proliferation has been detected on ESPIII surface.
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Authors thank to BAİBÜ-BAP Project division for financial supports (Project No: 2018.03.03.1295). Besides, Esra Cansever Mutlu thanks to BEYKENT UNIVERSITY BAP Project (Project No: 2018-19.BAP-18) for financial support. Also, this study was possible due to the benefit of the infrastructure of the National Centre for Micro and Nanomaterials as well as the National Centre for Food Security belonging to University Politehnica of Bucharest, Romania.
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Cansever Mutlu, E., Birinci Yıldırım, A., Yıldırım, M. et al. Improvement of antibacterial and biocompatibility properties of electrospray biopolymer films by ZnO and MCM-41. Polym. Bull. 77, 3657–3675 (2020). https://doi.org/10.1007/s00289-019-02937-2
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