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Prediction of breakthrough curves in packed-bed column as tool for lysozyme isolation using a green bed

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Abstract

The adsorption of lysozyme onto alginate–guar gum bed cross-linked with epichlorohydrin has been studied in packed-bed column. Adsorption performance was evaluated with different bed heights, flow rates and initial protein concentrations. The Thomas and bed depth service time mathematical models fitted well the breakthrough curves experimental data with high correlation coefficients. Not modification of the working yield of the bed was observed in progressive ten cycles of reused. Lysozyme recovery of 75% was achieved from white egg with a purification factor around 15 under the condition of bed height 9 cm, flow rate 0.4 mL/min and total protein inlet 10 mg/mL, while the dynamic bed capacity under this experimental condition was 10.87 mg/g hydrated bed.

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Abbreviations

GG:

Guar gum

Alg:

Alginate

LZ:

Lysozyme

Epi:

Epichlorohydrin

BT:

Breakthrough

Alg–GG:

Alginate–guar gum bed

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Acknowledgements

This work was supported by a grant from FonCyT, Project PICT 2013–271 – Argentina Innovator 2020. MEB is a fellowship from FonCyT.

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Authors and Affiliations

  1. Institute of Biotechnological and Chemistry Processes (IPROBYQ-CONICET) and Faculty of Biochemical and Pharmaceutical Sciences, National University of Rosario, Suipacha 570, S2002RLK, Rosario, Argentina

    M. Emilia Brassesco, Nadia Woitovich Valetti & Guillermo Picó

Authors
  1. M. Emilia Brassesco
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  2. Nadia Woitovich Valetti
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  3. Guillermo Picó
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Correspondence to Guillermo Picó.

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Brassesco, M.E., Woitovich Valetti, N. & Picó, G. Prediction of breakthrough curves in packed-bed column as tool for lysozyme isolation using a green bed. Polym. Bull. 76, 5831–5847 (2019). https://doi.org/10.1007/s00289-019-02683-5

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  • DOI: https://doi.org/10.1007/s00289-019-02683-5

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