Polymer Bulletin

, Volume 76, Issue 2, pp 701–724 | Cite as

Development of gelatin hydrogel pads incorporated with Eupatorium adenophorum essential oil as antibacterial wound dressing

  • P. ChuysinuanEmail author
  • N. Chimnoi
  • N. Reuk-Ngam
  • P. Khlaychan
  • A. Makarasen
  • N. Wetprasit
  • D. Dechtrirat
  • P. Supaphol
  • S. TechasakulEmail author
Original Paper


In this study, gelatin hydrogel was obtained by mixing a 10 wt% gelatin solution with essential oil extracted from Eupatorium adenophorum Spreng plant (Crofton weed). This study aimed to fabricate gelatin hydrogel pads containing 5–30% v/v Eupatorium adenophorum emulsion. Incorporation of emulsion into hydrogels caused increase in gel fraction because of a change in the cross-link capacity and decrease in Young’s modulus, levels of stress at maximum load, and fraction of strain at yield, dose-dependently. Furthermore, the swelling and weight loss properties of the tested hydrogels increased with an increase in the emulsion content. E. adenophorum emulsion-loaded gelatin hydrogel pads for patients with open wounds as a result of injury or surgical procedure were evaluated by analyzing the release profiles and the antimicrobial activity of the hydrogels. The emulsion-loaded hydrogel pads presented good antibacterial activity against the test pathogens. The composition of E. adenophorum essential oil was analyzed using GC–MS to further determine the antimicrobial activity of the essential oil. All the emulsion-loaded gelatin hydrogels inhibited the growth of the tested pathogens, namely Staphylococcus aureus, Staphylococcus epidermidis, Bacillus cereus, and Escherichia coli. Moreover, the antibacterial activity increased with the increase in initial amount of emulsion in the hydrogels. Thus, hydrogels show potential for use as antibacterial wound dressings.

Graphical Abstract


Gelatin Hydrogel Eupatorium adenophorum Antibacterial Essential oil 



The authors would like to acknowledge the financial support provided by the Thailand Research Fund (TRF; TRG5780204) and Chulabhorn Research Institute. This work was supported in part by the Research Pyramid, Radchadaphiseksomphot Endowment Fund (GCURP_58_02_63_01) of Chulalongkorn University.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • P. Chuysinuan
    • 1
    Email author
  • N. Chimnoi
    • 2
  • N. Reuk-Ngam
    • 1
  • P. Khlaychan
    • 1
  • A. Makarasen
    • 1
  • N. Wetprasit
    • 3
  • D. Dechtrirat
    • 4
  • P. Supaphol
    • 5
  • S. Techasakul
    • 1
    Email author
  1. 1.Laboratory of Organic SynthesisChulabhorn Research InstituteBangkokThailand
  2. 2.Laboratory of Natural ProductsChulabhorn Research InstituteBangkokThailand
  3. 3.Department of Biotechnology, Faculty of ScienceRamkhamhaeng UniversityBangkokThailand
  4. 4.Department of Materials Science, Faculty of ScienceKasetsart UniversityBangkokThailand
  5. 5.The Petroleum and Petrochemical CollegeChulalongkorn UniversityBangkokThailand

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