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Functionalization of poly(epichlorohydrin) using sodium hydrogen squarate: cytotoxicity and compatibility in blends with chitosan

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Abstract

Polymeric blends between chitosan and poly(epichlorohydrin) (PECH) modified with sodium hydrogen squarate were prepared by the casting method, using formic acid (85%) as the solvent. The compatibility of the blends was studied by different methods, such as Fourier transform infrared spectroscopy, Raman spectroscopy, and thermogravimetric analyses. Cytotoxicity assays were also performed by the direct contact method to assess the feasibility of using these materials in veterinary devices. Based on the analysis results, we concluded that these polymers are compatible due to the interaction between the NH3+ chitosan groups and CO− groups of the modified poly(epichlorohydrin), as well the hydrogen bond between the chlorine atom of the modified poly(epichlorohydrin) and the hydrogens of the chitosan methyl group. It is important to highlight that electrostatic interactions are also responsible for the non-toxicity of these materials in bovine fibroblast cells, which indicates the feasibility of their use in veterinary devices for cattle.

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Acknowledgements

The authors wish to thank CNPq, CAPES and FAPEMIG (Brazilian agencies) for financial support.

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Authors and Affiliations

  1. Department of Exact Science and Biotechnology, Federal University of Tocantins, Gurupi, TO, Brazil

    Nelson Luis G. D. Souza

  2. Department of Biology, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil

    Michele Munk

  3. Embrapa Dairy Cattle (CNPGL), Juiz de Fora, MG, Brazil

    Humberto M. Brandão

  4. Department of Chemistry, Federal University of Juiz de Fora, Juiz de Fora, MG, Brazil

    Luiz Fernando C. de Oliveira

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  1. Nelson Luis G. D. Souza
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Correspondence to Nelson Luis G. D. Souza.

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Souza, N.L.G.D., Munk, M., Brandão, H.M. et al. Functionalization of poly(epichlorohydrin) using sodium hydrogen squarate: cytotoxicity and compatibility in blends with chitosan. Polym. Bull. 75, 4627–4639 (2018). https://doi.org/10.1007/s00289-018-2290-5

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