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Fabrication and characterization of chitosan/gelatin/nanodiopside composite scaffolds for tissue engineering application

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Abstract

Composite scaffolds were prepared from the mixture of chitosan (C), gelatin (G) and nanodiopside (nDP) in different inorganic/organic weight ratios using the freeze-drying method. The prepared nDP and composite scaffolds were investigated using BET, FT-IR, SEM and XRD techniques. The composite scaffolds had 70–81% porosities with interlinked porous networks. Moreover, investigation of the cell proliferation, adhesion and viability using MTT test and mouse preosteoblast cell proved the cytocompatible nature of the composite scaffolds with improved cell attachment and proliferation. All these results essentially illustrated that this composite could have a potential ability for the tissue engineering applications.

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Acknowledgements

The authors are grateful to the Payame Noor University in Isfahan Research council (Grant # 62370). The authors would like to thank Dr. L. Ghorbanian for the critical reading of the manuscript, and Mrs. Hydari for providing cell culture facilities.

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Authors and Affiliations

  1. Department of Chemistry, Payame Noor University (PNU), P.O. Box 81395-671, Tehran, 19395-3697, Iran

    Abbas Teimouri, Shahin Roohafza & Mohammad Azadi

  2. Department of Chemistry, Isfahan University of Technology, Isfahan, 841543111, Iran

    Alireza Najafi Chermahini

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  1. Abbas Teimouri
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  2. Shahin Roohafza
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  4. Alireza Najafi Chermahini
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Correspondence to Abbas Teimouri.

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Teimouri, A., Roohafza, S., Azadi, M. et al. Fabrication and characterization of chitosan/gelatin/nanodiopside composite scaffolds for tissue engineering application. Polym. Bull. 75, 1487–1504 (2018). https://doi.org/10.1007/s00289-017-2096-x

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  • DOI: https://doi.org/10.1007/s00289-017-2096-x

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