Massilia aquatica sp. nov., Isolated from a Subtropical Stream in China


A Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and motile strain FT127WT was isolated from a subtropical stream in China. Comparison based on 16S rRNA gene sequences showed that strain FT127WT belongs to genus Massilia and shares 98.5% similarity with Massilia buxea A9T as its closest neighbor. The genome size of strain FT127WT was 6.65 Mbp with G + C content of 65.3%. The calculated pairwise OrthoANIu values and digital DNA–DNA hybridization values between strain FT127WT and each of strains M. buxea KCTC 52429T, Massilia armeniaca ZMN-3T, Massilia plicata DSM 17505T and Massilia namucuonensis CGMCC 1.11014T were less than 83.1% and 26.6%, respectively. The reconstructed phylogenomic tree based on concatenated 92 core genes showed that strain FT127WT clusters closely with M. namucuonensis CGMCC 1.11014T. The respiratory quinone of strain FT127WT was Q-8. The major fatty acids were C16:1ω7c, C16:0 and C12:0. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid. Combining above all characteristics, strain FT127WT should represent a novel species within genus Massilia, for which the name Massilia aquatica sp. nov. (type strain FT127WT = GDMCC 1.1690T = KACC 21482T) is proposed.

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This work was funded by Guangdong Provincial Programs for Science and Technology Development (2019B110205004; 2018B030324002), National Natural Science Foundation of China (91851202; 51678163), GDAS’ Special Project of Science and Technology Development (2019GDASYL-0301002; 2020GDASYL-20200402001). The reference strain M. armeniaca ZMN-3 T was kindly donated by Dr. Hai-Dong Huang (Tianjin Agricultural University, Tianjin 300384, PR China).

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Strain FT127WT was isolated by H-BL. Data collection and analysis were performed by H-BL and T-CD. The first draft of the manuscript was written by H-BL and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Mei-Ying Xu.

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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence and draft genome sequence of strain FT127WT are MN865813 and WWCU00000000, respectively.

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Lu, H., Deng, T. & Xu, M. Massilia aquatica sp. nov., Isolated from a Subtropical Stream in China. Curr Microbiol (2020).

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