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Flavihumibacter soli sp. nov., Isolated from Soil

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Abstract

A novel Gram-stain-positive, strictly aerobic, non-motile, rod-shaped bacterium, designated strain R14T was isolated from steep grove soil and taxonomically characterized using a polyphasic approach. Cells showed oxidase-negative and catalase-positive activities and grew at 25–37 °C (optimum, 30 °C), pH 5.0–8.0 (optimum, pH 6.5), and in the presence of 0–0.5% (w/v) NaCl (optimum, 0%). Phylogenetic trees based on 16S rRNA gene sequences revealed that strain R14T formed a phylogenetic lineage with Flavihumibacter genus members within the family Chitinophagaceae. Comparison of 16S rRNA gene sequences showed that strain R14T shared highest similarities with Flavihumibacter solisilvae 3-3T (97.5%), Flavihumibacter petaseus NBRC 106054T (96.7%), and Flavihumibacter stibioxidans YS-17T (96.1%). The G+C content of strain R14T calculated from its whole genome sequence was 44.6 mol%. Strain R14T contained menaquinone-7 and iso-C15:0, iso-C17:0 3-OH, C16:0, C15:1 ω6c, and iso-C15:0-G as the sole respiratory isoprenoid quinone and major cellular fatty acids, respectively. Major polar lipids consisted of phosphatidylethanolamine, two unidentified aminolipids, and four unidentified lipids. Based on phylogenetic, phenotypic, and chemotaxonomic characteristics of strain R14T, it could be concluded that strain R14T represents a novel species of the genus Flavihumibacter, for which the name Flavihumibacter soli sp. nov. is proposed. The type strain is R14T (=KACC 21229T = JCM 33473T).

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Acknowledgements

This work was supported by the National Research Foundation (2017M3C1B5019250) of the Ministry of Science and ICT and the National Institute of Biological Resources (NIBR202002203), funded by the Ministry of Environment (MOE), Republic of Korea.

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Authors and Affiliations

  1. Department of Life Science, Chung-Ang University, 84, HeukSeok-Ro, Dongjak-Gu, Seoul, 06974, Republic of Korea

    Ye Lin Seo, Jaejoon Jung, Shehzad Abid Khan, Kyung Hyun Kim & Che Ok Jeon

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  1. Ye Lin Seo
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  2. Jaejoon Jung
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  3. Shehzad Abid Khan
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  4. Kyung Hyun Kim
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Contributions

YLS isolated the type strain from soil and YLS and JJ performed the experiments. SAK and KHK analyzed the genome and constructed phylogenetic trees. COJ supervised all works. YLS and COJ wrote the manuscript. The manuscript has been reviewed and edited by all authors.

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Correspondence to Che Ok Jeon.

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The GenBank Accession Numbers for the 16S rRNA gene and genome sequences of strain R14T are MN086351 and JAAFZB000000000, respectively.

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Seo, Y.L., Jung, J., Khan, S.A. et al. Flavihumibacter soli sp. nov., Isolated from Soil. Curr Microbiol 77, 3179–3184 (2020). https://doi.org/10.1007/s00284-020-02103-2

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  • DOI: https://doi.org/10.1007/s00284-020-02103-2

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