Exploring the Genome of Fructobacillus tropaeoli CRL 2034, a Fig-Origin Strain that Produces High Levels of Mannitol from Fructose

Abstract

We report the draft genome sequence of Fructobacillus tropaeoli CRL 2034, a strain isolated from ripe fig in Tucumán province, Argentina. The interest in studying the genome of this fructophilic lactic acid bacterium strain was motivated by its ability to produce high levels of mannitol from fructose. This polyol has multiple industrial applications; however, it is mainly used as low calorie sugar in the food industry. The assembled genome of this strain consists of a 1.66-Mbp circular chromosome with 1465 coding sequences and a G+C content of 44.6%. The analysis of this genome supports the one step reaction of fructose reduction to mannitol by the mannitol 2-dehydrogenase enzyme, which together with a fructose permease, were identified as involved in mannitol synthesis. In addition, a phylogenetic analysis was performed including other Leuconostocaceae members to which the Fructobacillus genus belongs to; according to the 16S rRNA gene sequences, the strain CRL 2034 was located in the Fructobacillus clade. The present genome sequence could be useful to further elucidate regulatory processes of mannitol and other bioactive metabolites and to highlight the biotechnological potential of this fruit-origin Fructobacillus strain.

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Data Availability

The genome sequence of the F. tropaeoli CRL 2034 was deposited under GenBank accession number WNLV00000000.1, BioProject accession number PRJNA587125, and BioSample accession number SAMN13178779.

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Acknowledgements

This work was supported by grants Préstamo BID-PICT 2014-312 and 2011-0175 (CERELOMICS) from FONCyT and PIP 2014-003 from CONICET, both from Argentina.

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Correspondence to Fernanda Mozzi or Raúl R. Raya.

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Ruiz Rodríguez, L.G., Mohamed, F., Bleckwedel, J. et al. Exploring the Genome of Fructobacillus tropaeoli CRL 2034, a Fig-Origin Strain that Produces High Levels of Mannitol from Fructose. Curr Microbiol (2020). https://doi.org/10.1007/s00284-020-02102-3

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