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Hymenobacter pomorum sp. nov., Isolated from Apple Orchard Soil

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Abstract

A Gram-stain-negative, non-motile, rod-shaped bacterial strain, designated 9-2-1-1T, was isolated from apple orchard soil in Daegu, Republic of Korea. Comparative 16S rRNA gene sequence analysis showed that the isolate belongs to the family Cytophagaceae, Bacteroidetes and it is most closely related to Hymenobacter metalli A2-91T (97.8% similarity) and Hymenobacter marinus KJ035T (96.6%). Growth of strain 9-2-1-1T was observed at 4–30 °C, pH 6–8, and in the presence of 0–1.0% NaCl. The G+C content of the genomic DNA was 62.0 mol%. The predominant respiratory quinone of the isolate was MK-7; the major fatty acids were C15:0 iso (29.3%), C16:1ω5c (15.4%), C15:0 anteiso (12.5%), summed feature 3 (C16:1ω7c/C16:1ω6c; 12.3%), and C16:0 (10.6%); and the major polar lipid was phosphatidylethanolamine. The phenotypic and chemotaxonomic data supported the affiliation of strain 9-2-1-1T with the genus Hymenobacter. However, the DNA–DNA relatedness between the isolate and H. metalli and H. marinus were 31.3% and 24.7%, respectively. The DNA–DNA hybridization result and the differentiating phenotypic properties clearly indicate that strain 9-2-1-1T is the representative of a novel species in the genus Hymenobacter, for which the name Hymenobacter pomorum sp. nov. is proposed. The type strain is 9-2-1-1T (=KCTC 52740T = JCM 32193T).

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Acknowledgements

This work was supported by the Brain Pool Program (Grant No. 2018H1D3A2065415) through the National Research Foundation (NRF) funded by the Ministry of Science and ICT, Republic of Korea.

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Authors and Affiliations

  1. School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea

    Leonid N. Ten, Weilan Li, Seung-Yeol Lee & Hee-Young Jung

  2. Department of Horticultural Science, Kyungpook National University, Daegu, 41566, Republic of Korea

    In-Kyu Kang

  3. School of Food Science and Biotechnology/Food and Bio-Industry Research Institute, Kyungpook National University, Daegu, 41566, Republic of Korea

    Young-Je Cho

  4. Department of Bio and Environmental Technology, Seoul Women’s University, Seoul, 01797, Republic of Korea

    Myung Kyum Kim

  5. Institute of Plant Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea

    Seung-Yeol Lee & Hee-Young Jung

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Ten, L.N., Li, W., Lee, SY. et al. Hymenobacter pomorum sp. nov., Isolated from Apple Orchard Soil. Curr Microbiol 76, 117–123 (2019). https://doi.org/10.1007/s00284-018-1595-9

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