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In Vitro Formation of Dickeya zeae MS1 Biofilm

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Abstract

Bacterial soft rot caused by Dickeya zeae MS1 (Erwinia chrysanthemi) is one of the most devastating banana diseases worldwide. However, knowledge of the development and ecological interactions of D. zeae MS1 biofilm is limited. Here, we visualized the development and architecture of D. zeae MS1 biofilm using confocal laser scanning microscopy, and we evaluated the ability of D. zeae MS1 to form biofilms under different environmental conditions (carbon sources, temperatures, pH levels and mineral elements) using a microtiter plate assay. We found that the development of D. zeae MS1 biofilm could be categorized into four phases and that mature biofilm consisted of a highly organized architecture of both bacterial cells and a self-produced matrix of extracellular polysaccharides. Furthermore, sucrose was the most suitable carbon source for supporting the growth of biofilm cells and that 32 °C and pH 7.0 were the most favorable of the temperatures and pH levels examined. Meanwhile, the addition of Ca2+, Fe2+, K+ and Na+ enhanced the formation of biofilm in minimal medium cultures, whereas 2.5 mM Cu2+ and Mn2+ was inhibitory. A better understanding of biofilm formation under different environmental parameters will improve our knowledge of the growth kinetics of D. zeae MS1 biofilm.

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Acknowledgements

We would like to thank Dr. Aiping Xu (Public Monitoring Center for Agro-product of Guangdong Academy of Agricultural Science, Guangdong, Guangzhou) and Weina Zhang (Centre for Agrobiological Gene Research, Guangdong Academy of Agricultural Sciences, Guangdong, Guangzhou) for their assistance with confocal laser scanning microscopy.

Funding

This work was supported by the Natural Science Foundation of Guangdong Province (No. 2015A030312002); the Guangzhou Science and Technology Project (No. 2014J4500034); the President Foundation of the Guangdong Academy of Agricultural Sciences (No. 201515).

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Authors and Affiliations

  1. College of Agriculture, Guangxi University, 100 Daxue Road, Nanning, 530004, China

    Ning Huang, Zhongwen Wang & Birun Lin

  2. Guangdong Key Laboratory of High Technology for Plant Protection, Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou, China

    Xiaoming Pu, Jingxin Zhang, Huifang Shen, Qiyun Yang & Birun Lin

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  1. Ning Huang
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  2. Xiaoming Pu
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Correspondence to Birun Lin.

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Huang, N., Pu, X., Zhang, J. et al. In Vitro Formation of Dickeya zeae MS1 Biofilm. Curr Microbiol 76, 100–107 (2019). https://doi.org/10.1007/s00284-018-1593-y

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