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Various Phyllosphere and Soil Bacterial Communities of Natural Grasses and the Impact Factors in a Copper Tailings Dam

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Abstract

Copper mining caused severe damage to the ecological environment of mining areas. The combination of microbe and plant remediation has an application potential in improving the absorption and transformation efficiency of heavy metals. The phyllosphere is the largest biointerface on the planet, and bacteria are the dominant microbial inhabitants of the phyllosphere, believed to be critical to plant growth and health. This study investigated the phyllospheric and soil bacteria communities using high-throughput sequencing, and endophyte infection statuses of four natural grasses by toluidine blue heparin assay. Results showed variation in phyllospheric bacterial community structure. Gammaproteobacteria were the most abundant bacterial population. Bacilli were found in the phyllosphere of Bothriochloa ischaemum and Imperata cylindrica, while Clostridia were only found in Calamagrostis epigejos. Alphaproteobacteria were the dominant bacteria in soil. In addition, bacterial communities were influenced by endophytic infection statuses. Oxalobacteraceae was associated with soil carbon and sulfur. Enterobacteriaceae had negative correlation with the ratio of soil carbon and nitrogen, and had positive correlation with Cd content. These results offer useful insights into phyllospheric bacterial community variance in four different natural grasses in a copper tailings dam.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China under Grant No. 31600308, Shanxi Scholarship Council of China under Grant No. 2016-006, and Shanxi Province Science Foundation for Youths under Grant No. 201601D021101.

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Authors and Affiliations

  1. Institute of Loess Plateau, Shanxi University, Taiyuan, 030006, Shanxi, China

    Tong Jia, Rui-Hong Wang & Bao-Feng Chai

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  1. Tong Jia
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  2. Rui-Hong Wang
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  3. Bao-Feng Chai
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Correspondence to Tong Jia.

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Jia, T., Wang, RH. & Chai, BF. Various Phyllosphere and Soil Bacterial Communities of Natural Grasses and the Impact Factors in a Copper Tailings Dam. Curr Microbiol 76, 7–14 (2019). https://doi.org/10.1007/s00284-018-1575-0

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