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Transcriptional Regulation Between the Two Global Regulators RovA and CRP in Yersinia pestis biovar Microtus

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Abstract

Yersinia pestis is a dangerous bacterial pathogen that can cause plague. Both RovA and cyclic AMP receptor protein (cAMP-CRP) are required for regulating biofilm- and virulence-related genes in Y. pestis. In this study, the transcriptional regulation between RovA and cAMP-CRP were analyzed by using primer extension, quantitative RT-PCR, LacZ fusion, and electrophoretic mobility shift assay. The results indicated that RovA repressed crp transcription in an indirect manner, while that RovA had no regulatory action on cyaA at the transcriptional level. In addition, cAMP-CRP did not regulate the transcription of rovA. Taken together with our previous results, complex regulatory interactions of RovA, cAMP-CRP, and PhoP/PhoQ in Y. pestis were revealed, which would promote us gain deeper understanding about coordinative modulation of biofilm- and virulence-related regulator genes.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (81801984), and by the Natural Science Foundation of Hubei Province (2018CFB184), and by the Hubei Province health and family planning scientific research project (WJ2018H0070).

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Authors and Affiliations

  1. Department of Transfusion, Wuhan General Hospital of PLA, No. 627, Wuluolu, Wuchang, Wuhan, 430070, Hubei, China

    Lei Liu, Yinjuan Ding, Yaqiong Zheng, Liping Cai & Shangen Zheng

  2. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China

    Lei Liu & Haihong Fang

  3. School of Medicine, Jiangsu University, No. 301, Xuefulu, Jingkou, Zhenjiang, 212013, Jiangsu, China

    Yiquan Zhang

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  1. Lei Liu
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  2. Haihong Fang
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  4. Yaqiong Zheng
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Correspondence to Shangen Zheng or Yiquan Zhang.

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Liu, L., Fang, H., Ding, Y. et al. Transcriptional Regulation Between the Two Global Regulators RovA and CRP in Yersinia pestis biovar Microtus. Curr Microbiol 75, 1634–1641 (2018). https://doi.org/10.1007/s00284-018-1571-4

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  • DOI: https://doi.org/10.1007/s00284-018-1571-4

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