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Insight into Bacterial Community Diversity and Monthly Fluctuations of Medicago sativa Rhizosphere Soil in Response to Hydrogen Gas Using Illumina High-Throughput Sequencing

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Abstract

The microbial diversity and the monthly fluctuations in Medicago sativa field soil in response to hydrogen gas were investigated. Illumina high-throughput sequencing was used to analyze the bacteria in raw and hydrogen-treated rhizosphere soil. Among the 18 soil samples, the abundance change of the predominant phyla Proteobacteria and Actinobacteria showed opposite trends. The diversity index analysis of the nine leguminous soil samples showed the highest diversity of the microbial community in July. In the other nine soil samples treated with hydrogen, the microbial diversity decreased and the diversity of soil microorganisms in September was higher than that in July, but not significantly so. The heat map analysis revealed that the microbial community composition of the soil samples was different before and after the hydrogen treatment. After the soil samples were treated with hydrogen, the dominant genera were Nocardioide, Pseudomonas, Janibacter, Microbacterium, Microvirga, Streptomyces, and Phenylobacterium in May; Bradyrhizobium, Haliangium, Sphingomonas, Blastocatella, Lysobacter, and Sphingopyxis in July; and Aeromicrobium, Pseudonocardia, Lentzea, and Skermanella in September. This study indicates that time and hydrogen gas have significant effects on the diversity of microbes in M. sativa rhizospheric soil.

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Acknowledgements

This study is supported by the National Natural Science Foundation (Grant No. 41571243) and the project of young talents of science and technology association of Shaanxi Province (Project Number: 20160232). We thank Biomarker Technologies for their excellent technical assistance.

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Authors and Affiliations

  1. Key Laboratory of Resource Biology and Biotechnology in Western China, College of Life Sciences, Northwest University, Ministry of Education, Xi’an, Shaanxi, China

    Zhiying Li, Xin Liu, Ruirui Liu, Lulu Li, Lin Wang & Weiwei Wang

  2. Department of Medical Technology, Xi’an Medical University, Xi’an, Shaanxi, China

    Lin Wang

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  1. Zhiying Li
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  2. Xin Liu
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Correspondence to Weiwei Wang.

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Li, Z., Liu, X., Liu, R. et al. Insight into Bacterial Community Diversity and Monthly Fluctuations of Medicago sativa Rhizosphere Soil in Response to Hydrogen Gas Using Illumina High-Throughput Sequencing. Curr Microbiol 75, 1626–1633 (2018). https://doi.org/10.1007/s00284-018-1569-y

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