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Coralloluteibacterium thermophilus sp. nov., A Gammaproteobacterium Isolated from an Oil Reservoir

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Abstract

A Gram-negative, yellow-pigmented, aerobic bacterium, designated strain B51-30T, was isolated from oil-well production liquid in Baolige oilfield, China. The strain was able to grow at pH 6–10 (optimum at pH 7.5), in 0–6% (w/v) NaCl (optimum at 1%, w/v) at 15–55 °C (optimum at 45 °C). Cells of the isolate were non-motile and non-spore-forming rods. The major cellular fatty acids were iso-C15:0, iso-C11:0, iso-C11:0 3OH, iso-C17:1 ω9c, and iso-C17:0. Ubiquinone 8 was the predominant respiratory quinone. The major polar lipids consisted of phosphatidylethanolamine and diphosphatidylglycerol. The genomic DNA G+C content of the isolate was 70.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain B51-30T was most closely related to Coralloluteibacterium stylophorae KCTC 52167T (98.7% similarity). The two strains showed DNA–DNA relatedness values of 58.5%. Genotypic and phenotypic features indicate that strain B51-30T represents a novel species of the genus Coralloluteibacterium, for which the name Coralloluteibacterium thermophilus sp. nov. is proposed. The type strain is B51-30T (= CGMCC 1.13574T = KCTC 62780T).

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Acknowledgements

This work was supported by the China National Petroleum Corporation (Grant Number 2017E-1507) and the Huabei Oilfield Company (Grant Number 2017-HB-B19).

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Authors and Affiliations

  1. School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, China

    Jing You & Bozhong Mu

  2. Institute of Microbiology, CAS, Beijing, 100101, China

    Shan Hong, Bo Liu, Cheng Zhou & Yanfen Xue

  3. Engineering Technology Research Institute of Huabei Oilfield Company, Renqiu, 062552, China

    Jing You, Jiliang Yu & Lisha Duan

Authors
  1. Jing You
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  2. Shan Hong
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  3. Jiliang Yu
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  4. Lisha Duan
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  5. Bo Liu
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  6. Bozhong Mu
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  7. Cheng Zhou
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  8. Yanfen Xue
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Corresponding authors

Correspondence to Bozhong Mu or Cheng Zhou.

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Additional information

The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain B51-30T is MH174077 and the DPD number is TA00557.

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You, J., Hong, S., Yu, J. et al. Coralloluteibacterium thermophilus sp. nov., A Gammaproteobacterium Isolated from an Oil Reservoir. Curr Microbiol 75, 1584–1588 (2018). https://doi.org/10.1007/s00284-018-1564-3

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  • DOI: https://doi.org/10.1007/s00284-018-1564-3

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