A New Approach of Rpf Addition to Explore Bacterial Consortium for Enhanced Phenol Degradation Under High Salinity Conditions
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Only a small fraction of salt-tolerant phenol-degrading bacteria can be isolated by conventional plate separation methods, because most bacteria in nature are in a viable but non-culturable (VBNC) state. The aims of this study were to screen out more effective functional bacteria using resuscitation-promoting factor (Rpf), and to determine whether a mixed bacterial consortium possesses better phenol-degrading capabilities under high salinity conditions. The results indicated that three strains unique to treatment group with Rpf addition were obtained. A mixed bacterial consortium consisting of two high-efficient strains which belonged to genera Bacillus and Corynebacterium was capable of utilizing phenol as a sole source of carbon at high salinity. Complete degradation of 100 mg/L phenol at 2% NaCl concentration was achieved within 8 h. This study provides new insights into resuscitation of VBNC bacteria for enhanced treatment of phenol-laden saline wastewater.
We gratefully acknowledge the financial supports provided by the Special Fund for the Zhejiang Research Academy of Environmental Science (Grant No. 2017F30030), the National Natural Science Foundation of China (Grant No. 41701354), the Natural Science Foundation of Zhejiang Province of China (Grant No. LQ17D010002), and the Research Fund for the Doctoral Research of Key University (Grant No. ZZ323205020516001108).
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Conflict and interest
The authors declare that they have no conflict of interest.
- 19.Jin Y, Gan G, Yu X, Wu D, Zhang L, Yang N, Hu J, Liu Z, Zhang L, Hong H, Yan X, Liang Y, Ding L, Pan Y (2017) Isolation of viable but non-culturable bacteria from printing and dyeing wastewater bioreactor based on resuscitation promoting factor. Curr Microbiol 74(7):787–797CrossRefPubMedGoogle Scholar
- 35.Su XM, Liu YD, Hashmi MZ, Ding LX, Shen CF (2015) Culture-dependent and culture-independent characterization of potentially functional biphenyl-degrading bacterial community in response to extracellular organic matter from Micrococcus luteus. Microb Biotechnol 8(3):569–578CrossRefPubMedPubMedCentralGoogle Scholar
- 38.White J, Gilbert J, Hill G, Hill E, Huse SM, Weightman AJ, Mahenthiralingam E (2011) Culture-independent analysis of bacterial fuel contamination provides insight into the level of concordance with the standard industry practice of aerobic cultivation. Appl Environ Microbiol 77(13):4527–4538CrossRefPubMedPubMedCentralGoogle Scholar