Abstract
Intestinal microbiota plays a crucial role in preventing the colonization and invasion by pathogens, and disruption of microbiota may cause opportunistic infections and diseases. Pathogens often have strategies to escape from the colonization resistance mediated by microbiota, but whether they also modulate the microbiota composition is still a topic of investigation. In the present study, we addressed this question using an opportunistic pathogen, Klebsiella pneumoniae serotype K1, which is known to cause pyogenic liver abscess (KLA) in about 30% of mice. We examined the effect of K. pneumoniae infection on cecal microbiota composition by performing high-throughput 454 pyrosequencing of the hypervariable V3–V4 regions of bacterial 16S rRNA gene. Our data revealed that K. pneumoniae inoculation substantially changed the cecal microbiota composition when analyzed at the phylum, order, and family levels. Most strikingly, the KLA-infected mice had significantly increased abundance of Bacteroidales and Enterobacteriales and decreased abundance of Lactobacillales and Eggerthellales. Furthermore, by comparing the infected mice with or without KLA disease symptoms, we identified specific microbiota changes associated with the KLA disease induction. Especially, the KLA group had dramatically decreased sequence identical to Lactobacillus compared with non-KLA mice. These findings suggest that the pathogenic process of KLA infection may involve alteration of microbiota compositions, particularly reduction in Lactobacillus.
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Acknowledgements
We thank the support from Affiliated Hospital of Hebei University Postdoctoral workstation and Hebei University Biology Postdoctoral research station. We also thank the support from Key Bioengineering Discipline of Hebei Province.
Funding
This study was sported by Natural Science Foundation of Hebei Province (No. H2015201076), National Natural Science Foundation of China (No. 81000760), Medical Construction Special Project of Hebei University (No. 2014A2003).
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Nan Chen and Zong-Xin Ling have contributed equally to this work.
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284_2018_1471_MOESM1_ESM.tif
Richness estimators ACE (a) and Chao1 (b) and diversity indices Shannon (c) and Simpson (d) were calculated using mothur. (healthy vs KLA, P > 0.05; non-KLA vs KLA, P > 0.05). (TIF 5701 KB)
284_2018_1471_MOESM2_ESM.tif
Differences in gut microbiota taxon abundance between healthy and non-KLA groups. Comparison of relative abundance in bacterial sequences at the levels of phylum (a), order (b) and family (c) between healthy (Green) and non-KLA (Blue) groups; *P < 0.05. (TIF 7140 KB)
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Chen, N., Ling, ZX., Jin, TT. et al. Altered Profiles of Gut Microbiota in Klebsiella pneumoniae-Induced Pyogenic Liver Abscess. Curr Microbiol 75, 952–959 (2018). https://doi.org/10.1007/s00284-018-1471-7
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DOI: https://doi.org/10.1007/s00284-018-1471-7