Correlation Between Quorum Sensing Signal Molecules and Pseudomonas aeruginosa’s Biofilm Development and Virulency
Bacteria, when adhered to a substratum, can form biofilms. Nevertheless, many factors dictate biofilm formation and virulence factor production, including a response by the bacteria to their surroundings. This system is referred to as Quorum sensing (QS) also known as cell–cell communication. Pseudomonas aeruginosa is an infection causing agent in immune-compromised patients, it uses acyl-homoserine lactone (AHL) to coordinate its QS systems. In this work, the connection between some members of AHL produced by P. aeruginosa PAO1 and its biofilm development and the production of virulence factor was investigated. It was discovered that N-butanoyl-homoserine lactone (C4-HSL) and N-hexanoyl-l-homoserine lactone (C6-HSL) perform a more consequential and eminent function in the biofilm maturation and virulence factor production while N-(3-oxododecanoyl)-l-homoserine lactone (3OC12-HSL) plays a role in biofilm initiation. Because QS has been reported to be required for biofilm development and pathogenesis of P. aeruginosa, the results of this work have great importance and significance for the design of strategies for the control and prevention of biofilms.
This research was supported by a Grant (code 18IFIP-B087389-05) from the Industrial Facilities and Infrastructure Research Program funded by the Ministry of Land, Infrastructure, and Transport of Korean government.
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Conflict of interest
The authors declare no competing financial interest.
- 13.Stoodley P, Jørgensen F, Williams P, Lappin-Scott HM (1999) The role of hydrodynamics and AHL signalling molecules as determinants of the structure of Pseudomonas aeruginosa biofilms. In: Bayston R, Brading M, Gilbert P, Walker J, Wimpenny JWT (eds) Biofilms: the good, the bad, and the ugly. 4th meeting of the Biofilm Club. BioLine, Cardiff, GBGoogle Scholar
- 14.Heydorn A, Ersbøll B, Kato J, Hentzer M, Parsek MR, Tolker-Nielsen T, Givskov M, Molin S (2002) Statistical analysis of Pseudomonas aeruginosa biofilm development: impact of mutations in genes involved in twitching motility, cell-to-cell signaling, and stationary-phase sigma factor expression. Appl Environ Microbiol 68(4):2008–2017CrossRefPubMedPubMedCentralGoogle Scholar
- 20.Kurachi M (1958) Studies on the biosynthesis of pyocyanine. (II): isolation and determination of pyocyanine. Bull Inst Chem Res Kyoto Univ 36(6):174–187Google Scholar
- 21.Genigeorgis C (1995) Biofilm: their significance to cleaning in the meat sector. New challenges in meat hygiene: specific problems in cleaning and disinfection, Ecceamst, European Consortium for Continuing Education. Adv Meat Sci Technol 99:29–47Google Scholar