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Assessment of Multi Fragment Melting Analysis System (MFMAS) for the Identification of Food-Borne Yeasts

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Abstract

Multi Fragment Melting Analysis System (MFMAS) is a novel approach that was developed for the species-level identification of microorganisms. It is a software-assisted system that performs concurrent melting analysis of 8 different DNA fragments to obtain a fingerprint of each strain analyzed. The identification is performed according to the comparison of these fingerprints with the fingerprints of known yeast species recorded in a database to obtain the best possible match. In this study, applicability of the yeast version of the MFMAS (MFMAS-yeast) was evaluated for the identification of food-associated yeast species. For this purpose, in this study, a total of 145 yeast strains originated from foods and beverages and 19 standard yeast strains were tested. The DNAs isolated from these yeast strains were analyzed by the MFMAS, and their species were successfully identified with a similarity rate of 95% or higher. It was shown that the strains belonged to 43 different yeast species that are widely found in the foods. A clear discrimination was also observed in the phylogenetically related species. In conclusion, it might be suggested that the MFMAS-yeast seems to be a highly promising approach for a rapid, accurate, and one-step identification of the yeasts isolated from food products and/or their processing environments.

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Acknowledgements

This study was supported by The Scientific and Technological Research Council of Turkey (TÜBİTAK Project Number TOVAG-114O177), and the MFMAS is a patent-protected product of Erciyes Technopark Inc.

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Authors and Affiliations

  1. Food Engineering Department, Faculty of Engineering, Erciyes University, Kayseri, Turkey

    Zülal Kesmen, Mine E. Büyükkiraz, Esra Özbekar & Özge Kılıç

  2. Computer Engineering Department, Faculty of Engineering, Erciyes University, Kayseri, Turkey

    Mete Çelik & F. Özge Özkök

  3. Food Engineering Department, Faculty of Agriculture, Atatürk University, Erzurum, Turkey

    Bülent Çetin

  4. Department of Gastronomy and Culinary Arts, Gelisim University, Istanbul, Turkey

    Hasan Yetim

Authors
  1. Zülal Kesmen
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  2. Mine E. Büyükkiraz
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  3. Esra Özbekar
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  4. Mete Çelik
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  5. F. Özge Özkök
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  6. Özge Kılıç
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  7. Bülent Çetin
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  8. Hasan Yetim
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Correspondence to Zülal Kesmen.

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Kesmen, Z., Büyükkiraz, M.E., Özbekar, E. et al. Assessment of Multi Fragment Melting Analysis System (MFMAS) for the Identification of Food-Borne Yeasts. Curr Microbiol 75, 716–725 (2018). https://doi.org/10.1007/s00284-018-1437-9

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  • DOI: https://doi.org/10.1007/s00284-018-1437-9

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