Current Microbiology

, Volume 75, Issue 5, pp 624–629 | Cite as

Aeromicrobium panacisoli sp. nov. Isolated from Soil of Ginseng Cultivating Field

  • Muhammad Zubair Siddiqi
  • Soon Youl Lee
  • Kang Duk Choi
  • Wan-Taek Im


A Gram-positive, rod-shaped, non-spore-forming, and aerobic bacterium (Gsoil 137T) was isolated from soil of a ginseng field of Pocheon province in South Korea and subjected to a polyphasic approach in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity, strain Gsoil 137T was shown to belong to the family Nocardioidaceae and was closely related to Aeromicrobium ginsengisoli Gsoil 098T (96.7%), Aeromicrobium panaciterrae (96.7%), and Aeromicrobium halocynthiae JCM 15749T (96.6%). Being phylogenetic, it was most closely related to Aeromicrobium halocynthiae JCM 15749T. The G+C content of the genomic DNA was 70.3 mol%. The diagnostic diamino acid of the cell wall peptidoglycan was LL-diaminopimelic acid. The predominant menaquinone was menaquinone MK-8 (H4) and MK-7 (H4) was a minor compound. The major cellular fatty acids were C14:0, C16:0, C18:1 ω9c and summed feature 4 (C16:1 ω7c/C15:0 iso 2-OH). All these data supported the affiliation of strain Gsoil 137T to the genus Aeromicrobium. The results of physiological and biochemical tests enabled strain Gsoil 137T to be differentiated genotypically and phenotypically from currently known Aeromicrobium species. Therefore, strain Gsoil 137T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobium panacisoli sp. nov. is proposed. The type strain is Gsoil 137T (= KCTC 19130T = DSM 17940T = CCUG 52475T).



This research was supported by the project on survey and excavation of Korean indigenous species of the National Institute of Biological Resources (NIBR) under the Ministry of Environment and by the Intelligent Synthetic Biology Center of Global Frontier Project funded by the Ministry of Education, Science and Technology (2014M3A6A8066437).

Supplementary material

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Supplementary material 1 (DOC 54 KB)


  1. 1.
    Atlas RM (1993) Handbook of microbiological media. CRC Press, Boca RatonGoogle Scholar
  2. 2.
    Buck JD (1982) Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44:992–993PubMedPubMedCentralGoogle Scholar
  3. 3.
    Cappuccino JG, Sherman N (2002) Microbiology, a laboratory manual, 6th edn. Pearson Education, Inc., LondonGoogle Scholar
  4. 4.
    Cui YS, Im WT, Yin CR, Lee JS, Lee KC, Lee ST (2007) Aeromicrobium panaciterrae sp. nov., isolated from soil of a ginseng field in South Korea. Int J Syst Evol Microbiol 57:687–691CrossRefPubMedGoogle Scholar
  5. 5.
    Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791CrossRefPubMedGoogle Scholar
  6. 6.
    Fitch WM (1971) Toward defining the course of evolution: minimum change for a specified tree topology. Syst Zool 20:406–416CrossRefGoogle Scholar
  7. 7.
    Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98Google Scholar
  8. 8.
    Hiraishi A, Ueda Y, Ishihara J, Mori T (1996) Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42:457–469CrossRefGoogle Scholar
  9. 9.
    Im WT, Liu QM, Yang JE, Kim MS, Kim SY, Lee ST, Yi TH (2010) Panacagrimonas perspica gen. nov., sp. nov., a novel member of Gammaproteobacteria isolated from soil of a ginseng field. J Microbiol 48:262–266CrossRefPubMedGoogle Scholar
  10. 10.
    Kim MK, Park MJ, Im WT, Yang DC (2008) Aeromicrobium ginsengisoli sp. nov., isolated from a ginseng field. Int J Syst Evol Microbiol 58:2025–2030CrossRefPubMedGoogle Scholar
  11. 11.
    Kim SH, Yang HO, Sohn YC, Kwon HC (2010) Aeromicrobium halocynthiae sp. nov., a taurocholic acid-producing bacterium isolated from the marine ascidian Halocynthia roretzi. Int J Syst Evol Microbiol 60:2793–2798CrossRefPubMedGoogle Scholar
  12. 12.
    Kimura M (1983) The neutral theory of molecular evolution. Cambridge University Press, CambridgeCrossRefGoogle Scholar
  13. 13.
    Komagata K, Suzuki K (1987) Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–203CrossRefGoogle Scholar
  14. 14.
    Mesbah M, Premachandran U, Whitman WB (1989) Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167CrossRefGoogle Scholar
  15. 15.
    Miller ES, Woese CR, Brenner S (1991) Description of the erythromycin-producing bacterium Arthrobacter sp. strain NRRL B-3381 as Aeromicrobium erythreum gen. nov., sp. nov Int J Syst Bacteriol 41:363–368CrossRefPubMedGoogle Scholar
  16. 16.
    Moore DD, Dowhan D (1995) Preparation and analysis of DNA. In: Ausubel FW, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) Current protocols in molecular biology. Wiley, New York, pp 2–11Google Scholar
  17. 17.
    Niu L, Xiong M, Tang T, Song L, Hu X, Zhao M, Zhang K (2015) Aeromicrobium camelliae sp. nov., isolated from Pu’er tea. Int J Syst Evol Microbiol 65:4369–4373CrossRefPubMedGoogle Scholar
  18. 18.
    Ramasamy D, Kokcha S, Lagier JC, Nguyen TT, Raoult D, Fournier PE (2012) Genome sequence and description of Aeromicrobium massiliense sp. nov. Stand Genom Sci 7:246–257CrossRefGoogle Scholar
  19. 19.
    Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Bio Evol 4:406–425Google Scholar
  20. 20.
    Sasser M (1990) Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. MIDI Inc, NewarkGoogle Scholar
  21. 21.
    Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefPubMedPubMedCentralGoogle Scholar
  22. 22.
    Tamura T, Yokota A (1994) Transfer of Nocardioides fastidiosa Collins and Stackebrandt 1989 to the genus Aeromicrobium as Aeromicrobium fastidiosum comb. nov. Int J Syst Bacteriol 44:608–611CrossRefGoogle Scholar
  23. 23.
    Tang Y, Zhou G, Zhang L, Mao J, Luo X, Wang M, Fang C (2008) Aeromicrobium flavum sp. nov., isolated from air. Int J Syst Evol Microbiol 58:1860–1863CrossRefPubMedGoogle Scholar
  24. 24.
    Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882CrossRefPubMedPubMedCentralGoogle Scholar
  25. 25.
    Yoon JH, Lee CH, Oh TK (2005) Aeromicrobium alkaliterrae sp. nov., isolated from an alkaline soil, and emended description of the genus Aeromicrobium. Int J Syst Evol Microbiol 55:2171–2175CrossRefPubMedGoogle Scholar

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Authors and Affiliations

  1. 1.Department of BiotechnologyHankyong National UniversityAnseong-siRepublic of Korea
  2. 2.Center for Genetic Information, Graduate School of Bio and Information TechnologyHankyong National UniversityAnseong-siRepublic of Korea

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