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The Draft Genome Sequence of Thermophilic Thermoanaerobacterium thermosaccharolyticum M5 Capable of Directly Producing Butanol from Hemicellulose

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Abstract

A novel thermophilic and butanogenic Thermoanaerobacterium thermosaccharolyticum M5 was successfully isolated and characterized, which could produce butanol from hemicellulose via a unique ethanol–butanol (EB) pathway through consolidated bioprocessing (CBP). This represents the first wild-type bacterium which could produce butanol from hemicellulose via CBP under thermophilic conditions. The assembled draft genome of strain M5 is 2.64 Mp, which contains 2638 genes and 2465 protein-coding sequences with 33.90% G + C content. Among these annotated proteins, xylanases, xylosidases, and bifunctional alcohol/aldehyde dehydrogenase (AdhE) play key roles in the achievement of EB production from hemicellulose through CBP.

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Acknowledgements

This work was supported by the Jiangsu Province Natural Science Foundation for Youths (No. BK20170993), the National Natural Science Foundation of China (21706125, 21727818, 21706124, 31700092), and the Project of State Key Laboratory of Materials-Oriented Chemical Engineering (KL16-08).

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Authors and Affiliations

  1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu South Road 30#, Nanjing, 211800, People’s Republic of China

    Yujia Jiang, Jie Liu, Weiliang Dong, Wenming Zhang, Yan Fang, Jiangfeng Ma, Min Jiang & Fengxue Xin

  2. Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University, Nanjing, 211800, People’s Republic of China

    Weiliang Dong, Wenming Zhang, Yan Fang, Jiangfeng Ma, Min Jiang & Fengxue Xin

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  1. Yujia Jiang
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  2. Jie Liu
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  3. Weiliang Dong
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  4. Wenming Zhang
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  5. Yan Fang
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  6. Jiangfeng Ma
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  7. Min Jiang
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  8. Fengxue Xin
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Corresponding authors

Correspondence to Min Jiang or Fengxue Xin.

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The authors have declared there was no conflict of interest.

Additional information

Nucleotide Sequence Accession Numbers

This Whole Genome project has been deposited into GenBank under the accession no. NKHD00000000. The version described in this paper is version NKHD01000000.

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Jiang, Y., Liu, J., Dong, W. et al. The Draft Genome Sequence of Thermophilic Thermoanaerobacterium thermosaccharolyticum M5 Capable of Directly Producing Butanol from Hemicellulose. Curr Microbiol 75, 620–623 (2018). https://doi.org/10.1007/s00284-017-1425-5

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  • DOI: https://doi.org/10.1007/s00284-017-1425-5

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