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Current Microbiology

, Volume 75, Issue 5, pp 588–596 | Cite as

Bioprospection of Culturable Endophytic Fungi Associated with the Ornamental Plant Pachystachys lutea

  • Amanda da Silva Ribeiro
  • Julio Cesar Polonio
  • Alessandra Tenório Costa
  • Caroline Menicoze dos Santos
  • Sandro Augusto Rhoden
  • João Lúcio Azevedo
  • João Alencar Pamphile
Article

Abstract

Endophytes are fungi and bacteria that inhabit plant tissues without causing disease. Endophytes have characteristics that are important for the health of the plant and have been isolated from several plants of economic and medicinal interest but rarely from ornamental plants. The current study isolates and identifies endophytic fungi from the leaves of Pachystachys lutea and evaluates the antagonistic activity of these endophytes as well as cellulase production by the endophytes. Fungi were isolated by fragmentation from surface-disinfected leaves and were identified by the sequencing of the ITS gene and the genes coding for EF 1-α and β-tubulin followed by multilocus sequence analysis. Molecular taxonomic analysis revealed that 78% of the identified fungi belonged to the genus Diaporthe. We also identified strains belonging to the genera Colletotrichum, Phyllosticta, Xylaria, Nemania, and Alternaria. Most of the strains tested were able to inhibit the growth of pathogenic fungi, especially PL09 (Diaporthe sp.), which inhibited the growth of Colletotrichum sp., and PL03 (Diaporthe sp.), which inhibited the growth of Fusarium oxysporum. The production of cellulase ranged from 0.87 to 1.60 μmol/min. Foliar endophytic fungal isolates from P. lutea showed promising results for the in vitro control of plant pathogens and for cellulase production. This paper is the first report on culturable endophytic fungi isolated from the ornamental plant P. lutea.

Notes

Acknowledgements

The authors would like to thank CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) for the Master’s scholarship and CAPES/PNPD-UEM post-doctoral scholarship and CNPq—Conselho Nacional de Desenvolvimento Científico e Tecnológico—(311534/2014-7; 447265/2014-8) and the Fundação Araucária (276/2014) for financial support.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

284_2017_1421_MOESM1_ESM.pdf (1.6 mb)
Supplementary material 1 (PDF 1648 KB)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Amanda da Silva Ribeiro
    • 1
  • Julio Cesar Polonio
    • 1
  • Alessandra Tenório Costa
    • 1
  • Caroline Menicoze dos Santos
    • 1
  • Sandro Augusto Rhoden
    • 2
  • João Lúcio Azevedo
    • 3
  • João Alencar Pamphile
    • 1
  1. 1.Department of Biotechnology, Genetics and Cell BiologyState University of MaringaMaringáBrazil
  2. 2.Federal Institute of Santa CatarinaSão Francisco do SulBrazil
  3. 3.Department of GeneticsCollege of Agriculture Luiz de Queiroz (ESALQ/USP)PiracicabaBrazil

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