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Lifespan Extension of Caenorhabditis elegans by Butyricicoccus pullicaecorum and Megasphaera elsdenii with Probiotic Potential

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Abstract

Butyricicoccus pullicaecorum and Megasphaera elsdenii inhabit the human intestine and have probiotic potential. The aim of this study was to evaluate the effects of B. pullicaecorum and M. elsdenii on the lifespan of Caenorhabditis elegans. They significantly (P < 0.05) extended the lifespan of C. elegans compared with Escherichia coli OP50, a standard food for the worm. Analysis of age-related biomarkers such as lipofuscin, body size, and locomotory activity showed that they retarded aging. They all failed to extend the lifespan of daf-12 or dbl-1 loss-of-function C. elegans mutants compared with E. coli OP50-fed worms. However, the increase in lifespan was observed in daf-16, jnk-1, pmk-1, and skn-1 mutants. Moreover, they increased the resistance of C. elegans to a human pathogen, Salmonella typhimurium. In conclusion, B. pullicaecorum and M. elsdenii extend the lifespan of C. elegans via the transforming growth factor-beta (TGF-β) pathway associated with anti-inflammatory processes in the innate immune system.

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Acknowledgements

This study was supported by Korea University Grant (K1711191).

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Authors and Affiliations

  1. Department of Public Health Science (Brain Korea 21 PLUS Program), Graduate School, Korea University, Seoul, 136-701, Republic of Korea

    Gayeung Kwon, Jiyun Lee & Young-Hee Lim

  2. Department of Bio-Food Analysis and Processing, Bio-Campus Korea Polytechnic College, Nonsan, Chungnam, 32943, Republic of Korea

    Jong-Ho Koh

  3. School of Biosystem and Biomedical Science, College of Health Science, Korea University, Seoul, 136-701, Republic of Korea

    Young-Hee Lim

  4. Department of Laboratory Medicine, Guro Hospital, Korea University, Seoul, 152-703, Republic of Korea

    Young-Hee Lim

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  1. Gayeung Kwon
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Correspondence to Young-Hee Lim.

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Kwon, G., Lee, J., Koh, JH. et al. Lifespan Extension of Caenorhabditis elegans by Butyricicoccus pullicaecorum and Megasphaera elsdenii with Probiotic Potential. Curr Microbiol 75, 557–564 (2018). https://doi.org/10.1007/s00284-017-1416-6

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  • DOI: https://doi.org/10.1007/s00284-017-1416-6

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