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Secondary Metabolites and Their Biological Activity from Aspergillus aculeatus KKU-CT2

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Abstract

The bioactive compounds of the fungus Aspergillus aculeatus strain KKU-CT2, have been studied. The crude extracts from this fungus showed good antimicrobial activity against human pathogens, including Gram-positive and Gram-negative bacteria and yeast-like fungi. Its chemical components were isolated and purified by chromatographic methods. The structures of the secondary metabolites were elucidated by spectroscopic methods (IR, 1H, and 13C NMR). They were identified as ergosterol peroxide (1), secalonic acid D (2), secalonic acid F (3), variecolin (4), variecolactone (5), and ergosterol (6). Compounds 1 and 4–6 are reported for the first time as fungal metabolites from this species. Compound 1 displayed inhibitory effects on HSV-1 with an IC50 of 11.01 μg/ml. Compounds 3, 4, and 6 exhibited antimalarial activity against Plasmodium falciparum with IC50 of 1.03, 1.47, and 5.31 µg/ml, respectively. Additionally, all compounds from A. aculeatus KKU-CT2 showed unprecedented anticancer activities against human epidermoid carcinoma in the mouth (KB) (compounds 1–6), human breast cancer (MCF-7) (compounds 2, 4, and 5), and human lung cancer cells (NCI-H187) (compounds 1–4 and 6). These results suggest that secondary metabolites from A. aculeatus KKU-CT2 might be interesting for further derivatization, targeting diseases such as cancer.

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Acknowledgements

This work was supported by the Protein and Proteomics Research Center for Commercial and Industrial Purposes (ProCCI), Khon Kaen University. We are also grateful to Professor Dr. Somdej Kanokmedhakul, head of the Natural Products Research Unit, Department of Chemistry, and the Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Thailand, for providing the laboratory facilities for chemical elucidation of the bioactive compounds.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand

    Natanong Yodsing & Sophon Boonlue

  2. Graduate School, Khon Kaen University, Khon Kaen, 40002, Thailand

    Natanong Yodsing

  3. Natural Products Research Unit, Department of Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand

    Ratsami Lekphrom & Watchara Sangsopha

  4. Faculty of Agriculture, Tottori University, 4-101 Koyama-cho Minami, Tottori, 680-8553, Japan

    Tadanori Aimi

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  1. Natanong Yodsing
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  2. Ratsami Lekphrom
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  3. Watchara Sangsopha
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  4. Tadanori Aimi
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  5. Sophon Boonlue
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Correspondence to Sophon Boonlue.

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Yodsing, N., Lekphrom, R., Sangsopha, W. et al. Secondary Metabolites and Their Biological Activity from Aspergillus aculeatus KKU-CT2. Curr Microbiol 75, 513–518 (2018). https://doi.org/10.1007/s00284-017-1411-y

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  • DOI: https://doi.org/10.1007/s00284-017-1411-y

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