Current Microbiology

, Volume 75, Issue 1, pp 79–83 | Cite as

Anthropogenic Activities Induce Depletion in Microbial Communities at Urban Sites of the River Ganges

  • Kunal Jani
  • Vikas Ghattargi
  • Shrikant Pawar
  • Mitali Inamdar
  • Yogesh Shouche
  • Avinash Sharma
Article

Abstract

The Ganges is the largest river of India, worshiped by Hindus with a belief that bathing in the river causes the remission of sins and is considered very pure. It is heavily polluted by the unrestricted human usage including ritual practices, urbanization, and industrialization. Such perturbations may subsequently influence the bacterial community composition and ecosystem functioning. Here, we applied targeted amplicon sequencing to determine the impact of spatial variation on the microbial community assemblage of the Ganga River. The river bacterial community demonstrates taxonomic variability across the sites with accumulation of Firmicutes (20.9%) Verrucomicrobia (6.09%), Actinobacteria (4.51%), and Synergistetes (1.16%), at rural site while Proteobacteria (49.4%) and Bacteroidetes (12.7%) predominate at urban sites. Furthermore, sites under study establish the unique taxonomic signature which could represent the impact of spatial variation on the microbial community assemblage.

Notes

Acknowledgements

This work was supported by the “Department of Biotechnology (DBT), Government of India” (by Grant No. BT/PR/0054/NDB/52/94/2007), under the Project “Establishment of Microbial Culture Collection’. We are grateful to Center for Citizen Science for their support in sample collection.

Compliance with Ethical Standards

Conflict of interest

All other authors declared no competing interests.

Supplementary material

284_2017_1352_MOESM1_ESM.docx (26 kb)
Supplementary material 1 (DOCX 26 kb)

References

  1. 1.
    Azam F, Worden AZ (2004) Oceanography. Microbes, molecules, and marine ecosystems. Science 303(5664):1622–1624CrossRefPubMedGoogle Scholar
  2. 2.
    Bartram AK, Lynch MDJ, Stearns JC et al (2011) Generation of multimillion-sequence 16S rRNA gene libraries from complex microbial communities by assembling paired-end Illumina reads. Appl Environ Microbiol 77:3846–3852CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, Fierer N, Pena AG, Goodrich JK, Gordon JI et al (2010) QIIME allows analysis of high-throughput community sequencing data. Nat Methods 7(5):335–336CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Central Pollution Control Board, Ministry of Environment and Forests, Govt. Of India (2013).Google Scholar
  5. 5.
    Correll DL (1998) The role of phosphorus in the eutrophication of receiving waters: a review. J Environ Qual 27:261–266CrossRefGoogle Scholar
  6. 6.
    David S, Roy N (2016) Public health perspectives from the biggest human mass gathering on earth: Kumbh Mela, India. Int J Infect Dis 47:42–45CrossRefPubMedGoogle Scholar
  7. 7.
    Feris KP, Ramsey PW, Gibbons SM, Frazar C, Rillig MC et al (2009) Hyporheic microbial community development is a sensitive indicator of metal contamination. Environ Sci Technol 43:6158–6163CrossRefPubMedGoogle Scholar
  8. 8.
    Jin L, Whitehead PG, Sarkar S, Sinha R, Futter MN, Butterfield D, Caesarf J, Crossmangh J (2015) Assessing the impacts of climate change and socioeconomic changes on flow and phosphorus flux in the Ganga river system. Environ Sci Process Impacts 17:1098–1110CrossRefPubMedGoogle Scholar
  9. 9.
    Kumbhare SV, Dhotre DP, Dhar SK et al (2015) Insights into diversity and imputed metabolic potential of bacterial communities in the continental shelf of Agatti Island. PLoS ONE 10(6):e0129864CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Langille MGI, Zaneveld J, Caporaso JG et al (2013) Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Nat Biotechnol 31:814–821CrossRefPubMedPubMedCentralGoogle Scholar
  11. 11.
    Magoč T, Salzberg SL (2011) FLASH: fast length adjustment of short reads to improve genome assemblies. Bioinformatics 27:2957–2963CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Ramsey PW, Rillig MC, Feris KP, Gordon NS, Moore JN et al (2005) Relationship between communities and processes; new insights from a field study of a contaminated ecosystem. Ecol Lett 8:1201–1210CrossRefPubMedGoogle Scholar
  13. 13.
    Sharma A, Paul D, Dhotre D et al (2017) Deep sequencing analysis of bacterial community structure of Soldhar hot spring, India. Microbiology 86:136–142CrossRefGoogle Scholar
  14. 14.
    Vaseem H, Banerjee TK (2016) Evaluation of pollution of Ganga River water using fish as bioindicator. Environ Monit Assess 188(8):444CrossRefPubMedGoogle Scholar
  15. 15.
    Verghese BG, Iyer RR (1993) Harnessing the Eastern Himalayan Rivers: Regional Cooperation in South Asia. Konark Publishers, New DelhiGoogle Scholar
  16. 16.
    Wang Y, Sheng HF, He Y, Wu JY, Jiang YX, Tam NFY, Zhoua HW (2012) Comparison of the levels of bacterial diversity in freshwater, intertidal wetland, and marine sediments by using millions of illumina tags. Appl Environ Microbiol 78(23):8264–8271CrossRefPubMedPubMedCentralGoogle Scholar
  17. 17.
    Whitehead PG, Barbour E, Futter MN, Sarkar S, Rodda H, Caesar J, Butterfield D, Sinha R, Nicholls R, Hutton C, Leckie HD (2015) Impacts of climate change and socio-economic scenarios on flow and water quality of the Ganges, Brahmaputra and Meghna (GBM) river systems: low flow and flood statistics. Environ Sci 17:1057–1069Google Scholar
  18. 18.
    Yadav A, Pandey J (2017) Water quality interaction with alkaline phosphatase in the ganga river: implications for river health. Bull Environ Contam Toxicol 99(1):75–82CrossRefPubMedGoogle Scholar
  19. 19.
    Zeglin LH, Dahm CN, Barrett JE, Gooseff MN, Fitpatrick SK et al (2011) Bacterial community structure along moisture gradients in the parafluvial sediments of two ephemeral desert streams. Microb Ecol 61:543–556CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  1. 1.National Centre for Microbial Resource, National Centre for Cell SciencePuneIndia
  2. 2.Center for Citizen SciencePuneIndia

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