Current Microbiology

, Volume 75, Issue 1, pp 20–26 | Cite as

Proteomic Analysis of Vibrio parahaemolyticus Under Cold Stress

  • Jing Tang
  • Juntao Jia
  • Ying Chen
  • Xiaohua Huang
  • Xiaoliang Zhang
  • Liqing Zhao
  • Wei Hu
  • Changjun Wang
  • Chao Lin
  • Zhenxing Wu


Vibrio parahaemolyticus is a kind of food-borne pathogenic bacterium, which can seriously infect food, especially seafood causing gastroenteritis and other disease. We studied the global proteome responses of V. parahaemolyticus under cold stress by nano-liquid chromatography-tandem mass spectrometry to improve the present understanding of V. parahaemolyticus proteomics events under cold stress. A total of 1151 proteins were identified and 101 proteins were differentially expressed, of which 69 were significantly up-regulated and 32 were downregulated. Functional categorization of these proteins revealed distinct differences between cold-stressed and control cells. These proteins were grouped into 21 functional categories by the clusters of orthologous groups (COG) analysis. The most of up-regulated proteins were functionally categorized as nucleotide transport and metabolism, transcription, function unknown, and defense mechanisms. These up-regulated proteins play an important role under cold stress.



We thank Dr. Mingwei Liu (Beijing Proteome Research Center) for sample preparation and Mass Spectrometry analysis. This work was supported by the Chinese State High-Tech Development Plan (2012AA101605), the Science Foundation of General Administration of Quality Supervision, Inspection, and Quarantine of the People’s Republic of China (2012IK305, 2013IK175, and 2016IK198).

Supplementary material

284_2017_1345_MOESM1_ESM.xlsx (115 kb)
Supplementary material 1 (XLSX 115 kb)


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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Jing Tang
    • 1
  • Juntao Jia
    • 1
  • Ying Chen
    • 2
  • Xiaohua Huang
    • 1
  • Xiaoliang Zhang
    • 1
  • Liqing Zhao
    • 1
  • Wei Hu
    • 1
  • Changjun Wang
    • 1
  • Chao Lin
    • 1
  • Zhenxing Wu
    • 1
  1. 1.Shandong Entry-Exit Inspection and Quarantine BureauQingdaoPeople’s Republic of China
  2. 2.Chinese Academy of Inspection and QuarantineBeijingChina

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