Abstract
Vibrio parahaemolyticus is a kind of food-borne pathogenic bacterium, which can seriously infect food, especially seafood causing gastroenteritis and other disease. We studied the global proteome responses of V. parahaemolyticus under cold stress by nano-liquid chromatography-tandem mass spectrometry to improve the present understanding of V. parahaemolyticus proteomics events under cold stress. A total of 1151 proteins were identified and 101 proteins were differentially expressed, of which 69 were significantly up-regulated and 32 were downregulated. Functional categorization of these proteins revealed distinct differences between cold-stressed and control cells. These proteins were grouped into 21 functional categories by the clusters of orthologous groups (COG) analysis. The most of up-regulated proteins were functionally categorized as nucleotide transport and metabolism, transcription, function unknown, and defense mechanisms. These up-regulated proteins play an important role under cold stress.
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Acknowledgements
We thank Dr. Mingwei Liu (Beijing Proteome Research Center) for sample preparation and Mass Spectrometry analysis. This work was supported by the Chinese State High-Tech Development Plan (2012AA101605), the Science Foundation of General Administration of Quality Supervision, Inspection, and Quarantine of the People’s Republic of China (2012IK305, 2013IK175, and 2016IK198).
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Tang, J., Jia, J., Chen, Y. et al. Proteomic Analysis of Vibrio parahaemolyticus Under Cold Stress. Curr Microbiol 75, 20–26 (2018). https://doi.org/10.1007/s00284-017-1345-4
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DOI: https://doi.org/10.1007/s00284-017-1345-4