Current Microbiology

, Volume 75, Issue 1, pp 6–10 | Cite as

Halobium Salinum sp. nov., Isolated from a Marine Solar Saltern

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Abstract

A halophilic archaeal strain YJ-8-ST was isolated from Yangjiang marine solar saltern, China. Cells from strain YJ-8-ST were pleomorphic, lysed in distilled water, stained Gram-negative, and formed red-pigmented colonies on agar plate. Optimal growth of the strain was obtained at 3.1 M NaCl (range 1.4–4.8 M), 0.1 M MgCl2 (range 0.005–1.0 M), 37 °C (range 20–50 °C), and pH 7.5 (range 5.5–9.5). The major polar lipids are phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), sulfated mannosyl glucosyl diether (S-DGD-1), and mannosyl glucosyl diether (DGD-1). The 16S rRNA gene and rpoB′ gene of strain YJ-8-ST were phylogenetically related to the corresponding genes of Halobium palmae (96.9–97.2 and 92.7% similarities, respectively). The DNA G+C content of strain YJ-8-ST was determined to be 68.9 mol%. The phenotypic, chemotaxonomic, and phylogenetic characteristics suggested that strain YJ-8-ST (=CGMCC 1.12553T = JCM 30029T) represents a new species of Halobium, for which the name Halobium salinum sp. nov. is proposed.

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31370054) and the 11th “Six Talents Peak” Project of Jiangsu Province (No. 2014-SWYY-021). We are grateful to Dr. Koji Mori (NITE Biological Resource Center, Japan) for kindly providing the type strain (Halobium palmae NBRC 111368T).

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Conflict of interest

The authors declare that no conflict of interest exists.

Supplementary material

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Supplementary material 1 (PDF 424 kb)

References

  1. 1.
    Cui H-L, Zhou P-J, Oren A, Liu S-J (2009) Intraspecific polymorphism of 16S rRNA genes in two halophilic archaeal genera, Haloarcula and Halomicrobium. Extremophiles 13:31–37CrossRefPubMedGoogle Scholar
  2. 2.
    Cui H-L, Gao X, Yang X, Xu X-W (2010) Halorussus rarus gen. nov., sp. nov., a new member of the family Halobacteriaceae isolated from a marine solar saltern. Extremophiles 14:493–499CrossRefPubMedGoogle Scholar
  3. 3.
    Dussault HP (1955) An improved technique for staining red halophilic bacteria. J Bacteriol 70:484–485PubMedPubMedCentralGoogle Scholar
  4. 4.
    Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376CrossRefPubMedGoogle Scholar
  5. 5.
    Fitch WM (1971) Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416CrossRefGoogle Scholar
  6. 6.
    Gupta RS, Naushad S, Baker S (2015) Phylogenomic analyses and molecular signatures for the class Halobacteria and its two major clades: a proposal for division of the class Halobacteria into an emended order Halobacteriales and two new orders, Haloferacales ord. nov. and Natrialbales ord. nov., containing the novel families Haloferacaceae fam. nov. and Natrialbaceae fam. nov. Int J Syst Evol Microbiol 65:1050–1069CrossRefPubMedGoogle Scholar
  7. 7.
    Gupta RS, Naushad S, Fabros R, Adeolu M (2016) A phylogenomic reappraisal of family-level divisions within the class Halobacteria: proposal to divide the order Halobacteriales into the families Halobacteriaceae, Haloarculaceae fam. nov., and Halococcaceae fam. nov., and the order Haloferacales into the families, Haloferacaceae and Halorubraceae fam nov. Antonie Van Leeuwenhoek 109:565–587CrossRefPubMedGoogle Scholar
  8. 8.
    Ivanov VM (2004) The 125th anniversary of the Griess reagent. J Anal Chem 59:1002–1005CrossRefGoogle Scholar
  9. 9.
    Kim O-S, Cho Y-J, Lee K, Yoon S-H, Kim M, Na H, Park S-C, Jeon YS, Lee J-H, Yi H, Won S, Chun J (2012) Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721CrossRefPubMedGoogle Scholar
  10. 10.
    Marmur J, Doty P (1962) Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118CrossRefPubMedGoogle Scholar
  11. 11.
    McDade JJ, Weaver RH (1959) Rapid methods for the detection of gelatin hydrolysis. J Bacteriol 77:60–64PubMedPubMedCentralGoogle Scholar
  12. 12.
    Minegishi H, Kamekura M, Itoh T, Echigo A, Usami R, Hashimoto T (2010) Further refinement of the phylogeny of the Halobacteriaceae based on the full-length RNA polymerase subunit B’ (rpoB′) gene. Int J Syst Evol Microbiol 60:2398–2408CrossRefPubMedGoogle Scholar
  13. 13.
    Mori K, Nurcahyanto DA, Kawasaki H, Lisdiyanti P, Yopi Y, Suzuki K-I (2016) Halobium palmae gen. nov. sp. nov. an extremely halophilic archaeon isolated from a solar saltern. Int J Syst Evol Microbiol 66:3799–3804CrossRefPubMedGoogle Scholar
  14. 14.
    Oren A, Ventosa A, Grant WD (1997) Proposed minimal standards for description of new taxa in the order Halobacteriales. Int J Syst Bacteriol 47:233–238CrossRefGoogle Scholar
  15. 15.
    Oren A (2014) Taxonomy of halophilic Archaea: current status and future challenges. Extremophiles 18:825–834CrossRefPubMedGoogle Scholar
  16. 16.
    Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425PubMedGoogle Scholar
  17. 17.
    Stackebrandt E, Ebers J (2006) Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33:152–155Google Scholar
  18. 18.
    Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefPubMedPubMedCentralGoogle Scholar
  19. 19.
    Wang Z, Xu J-Q, Xu W-M, Li Y, Zhou Y, Lü Z-Z, Hou J, Zhu L, Cui H-L (2016) Salinigranum salinum sp. nov., isolated from a marine solar saltern. Int J Syst Evol Microbiol 66:3017–3021CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Yang Li
    • 1
  • Yao Zhou
    • 1
  • Zhen-Zhen Lü
    • 1
  • Heng-Lin Cui
    • 1
  1. 1.School of Food and Biological EngineeringJiangsu UniversityZhenjiangPeople’s Republic of China

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