Halobium Salinum sp. nov., Isolated from a Marine Solar Saltern
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A halophilic archaeal strain YJ-8-ST was isolated from Yangjiang marine solar saltern, China. Cells from strain YJ-8-ST were pleomorphic, lysed in distilled water, stained Gram-negative, and formed red-pigmented colonies on agar plate. Optimal growth of the strain was obtained at 3.1 M NaCl (range 1.4–4.8 M), 0.1 M MgCl2 (range 0.005–1.0 M), 37 °C (range 20–50 °C), and pH 7.5 (range 5.5–9.5). The major polar lipids are phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), sulfated mannosyl glucosyl diether (S-DGD-1), and mannosyl glucosyl diether (DGD-1). The 16S rRNA gene and rpoB′ gene of strain YJ-8-ST were phylogenetically related to the corresponding genes of Halobium palmae (96.9–97.2 and 92.7% similarities, respectively). The DNA G+C content of strain YJ-8-ST was determined to be 68.9 mol%. The phenotypic, chemotaxonomic, and phylogenetic characteristics suggested that strain YJ-8-ST (=CGMCC 1.12553T = JCM 30029T) represents a new species of Halobium, for which the name Halobium salinum sp. nov. is proposed.
This work was supported by the National Natural Science Foundation of China (No. 31370054) and the 11th “Six Talents Peak” Project of Jiangsu Province (No. 2014-SWYY-021). We are grateful to Dr. Koji Mori (NITE Biological Resource Center, Japan) for kindly providing the type strain (Halobium palmae NBRC 111368T).
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The authors declare that no conflict of interest exists.
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