Abstract
It was found in a previous study that Mycobacterium tuberculosis protein tyrosine phosphatase ptpAt promoter is a highly active promoter in slow-growing species of mycobacteria, such as M. tuberculosis and M. bovis BCG, but inert in fast-growing mycobacterial species, such as M. smegmatis. This difference is presumed to be due to the differences between sigma factors systems of slow-growing pathogenic mycobacteria and the fast-growing saprophyte M. smegmatis. Therefore, we constructed a series of plasmids, named pOLYG-13x, which can express various M. tuberculosis sigma factors and also contain a PptpAt-gfp reporter gene construct. By inducing different sigma factor genes of M. tuberculosis in M. smegmatis, we were able to explore the influences of various sigma factors on the expression efficiency of the ptpAt promoter. The result show that of the 10 sigma factors evaluated, only sigF and sigL were able to weakly drive the ptpAt promoter in M. smegmatis and other sigma factors were unable to drive the promoter.
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Notes
The ptpA genes of M. tuberculosis and M. smegmatis are highly homologous. Here, M. tuberculos is ptpA is referred to as ptpAt, and M. smegmatis ptpA is referred to as ptpAs.
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Acknowledgments
This investigation received support from the 863 high-tech research and development program of China (2004AA2152021) as well as the Science and Technology Committee of Shanghai (02DJ14002). We are grateful to Prof. Yuhui Zhang for providing the pOLYG plasmid.
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Lei, J., Zhang, H., Wu, C. et al. The Influence of Mycobacterium tuberculosis Sigma Factors on the Promotion Efficiency of ptpAt Promoter in Mycobacterium smegmatis. Curr Microbiol 51, 141–147 (2005). https://doi.org/10.1007/s00284-005-7444-7
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DOI: https://doi.org/10.1007/s00284-005-7444-7