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Detection of Helicobacter Species in the Gastrointestinal Tract of Wild Rodents From Brazil

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Abstract

Since we have limited knowledge about the occurrence of Helicobacter in wild animals, we searched for Helicobacter species in the gastrointestinal tract of 75 rodents captured in forest remnants of Minas Gerais, Brazil. Fragments from the antrum and corpus of the stomach and from the colon were taken for PCR assays for Helicobacter detection. Although gastric mucosa was Helicobacter-positive in only one animal, the bacterium was detected in the colonic mucosa of 23 rodents (30.7%). Helicobacter detection was more frequent in the colon of terraced rice rat (56%) and house rat (30%) in contrast to punare and Spix’s yellow-toothed cavy, in which the presence of the bacterium was not detected. Helicobacter rodentium, H. marmotae, H. cinaedi, and other species closely related to the murine helicobacters were presumptively identified by DNA sequencing. Wild rodents may serve as a reservoir of these Helicobacter species in nature.

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Literature cited

  1. Fox JG (2002) The non-H. pylori helicobacters: their expanding role in gastrointestinal and systemic diseases. Gut 50:273–283

    Article  PubMed  CAS  Google Scholar 

  2. On SLV, Hynes S, Wadstrom T (2002) Extragastric Helicobacter species. Helicobacter 7(Suppl 1):63–67

    Article  PubMed  Google Scholar 

  3. Solnick JV, (2003) Clinical significance of Helicobacter species other than Helicobacter pylori. Clin Infect Dis 36:349–354

    Article  PubMed  Google Scholar 

  4. Weisbroth SH (1999) The rodent Helicobacters: Present status of diagnostic detection and guidelines for institutional procurement standards. Lab Anim 28:41–45

    Google Scholar 

  5. Fox JG, Dewhirst FE, Tully JG, et al. (1994) Helicobacter hepaticus sp. nov., a microaerophilic bacterium isolated from livers and intestinal mucosal scrapings from mice. J Clin Microbiol 32:1238–1245

    PubMed  CAS  Google Scholar 

  6. Fox JG, Yan LL, Dewhirst FE, et al. (1995) Helicobacter bilis sp. nov., a novel Helicobacter isolated from bile, livers, and intestines of aged, inbred mouse strains. J Clin Microbiol 33:445–454

    PubMed  CAS  Google Scholar 

  7. Shames B, Fox JG, Dewhirst F, et al. (1995) Identification of widespread Helicobacter hepaticus infection in feces in commercial mouse colonies by culture and PCR assay. J Clin Microbiol 33:2968–2072

    PubMed  CAS  Google Scholar 

  8. Franklin CL, Riley LK, Livingston RS, et al. (1999) Enterohepatic lesion in SCID mice infected with Helicobacter bilis. Lab Anim Sci 48:334–339

    Google Scholar 

  9. Cimolai N, Gill MJ, Jones AB (1987) “Campylobacter cinaedi” bacteremia: case report and laboratory findings. J Clin Microbiol 25:942–943

    PubMed  CAS  Google Scholar 

  10. Orliced SL, Welch DF, Kuhls RL (1993) Septicemia and meningitis caused by Helicobacter cinaedi in a neonate. J Clin Microbiol 31:569–571

    Google Scholar 

  11. Tee W, Leder K, Karroum E, et al. (1998) “Flexispira rappini” bacteremia in a child with pneumonia. J Clin Microbiol 36:1679–1682

    PubMed  CAS  Google Scholar 

  12. Giusti AM, Crippa L, Bellini O, et al. (1998) Gastric spiral bacteria in wild rats from Italy. J Wildl Dis 34:68–72

    Google Scholar 

  13. Won YS, Yoon JH, Lee CH, et al. (2002) Helicobacter muricola sp. nov., a novel Helicobacter species isolated from the ceca an feces of Korean wild mouse (Mus musculus molossinus). FEMS Microbiol Lett 209:45–51

    Article  PubMed  CAS  Google Scholar 

  14. Goto K, Jiang W, Zheng Q, et al. (2004) Epidemiology of Helicobacter infection in wild rodents in the Xinjiang-Uygur autonomous region of China. Curr Microbiol 49:221–223

    Article  PubMed  CAS  Google Scholar 

  15. Monteiro L, Grãs N, Vidal R, et al. (2001) Detection of Helicobacter pylori DNA in human feces by PCR: DNA stability and removal of inhibitors. J Microbiol Methods 45:89–94

    Article  PubMed  CAS  Google Scholar 

  16. Fox JG, Dewhirst FE, Shen Z, et al. (1998) Hepatic Helicobacter species identified in bile and gallbladder tissue from Chileans with chronic cholecystitis. Gastroenterology 114:755–763

    Article  PubMed  CAS  Google Scholar 

  17. Nowak RM (1999) Walker’s Mammals of the World. 6th edn. Baltimore, Johns Hopkins University Press

    Google Scholar 

  18. Queiroz DMM, Contigli C, Coimbra RS, et al. (1992) Spiral bacterium associated with gastric, ileal and caecal mucosa of mice. Lab Anim 26:288–294

    PubMed  CAS  Google Scholar 

  19. Patterson MM, Schrenzel MD, Feng Y, et al. (2000) Helicobacter aurati sp. nov., a urease-positive Helicobacter species cultured from gastrointestinal tissues of Syrian hamsters. J Clin Microbiol 38:3722–3728

    PubMed  CAS  Google Scholar 

  20. Moura SB, Queiroz DMM, Mendes EM, et al. (1993) The inflammatory response of the gastric mucosa of mice experimentally infected with “Gastrospirillum suis”. J Med Microbiol 39:64–68

    Article  PubMed  CAS  Google Scholar 

  21. Mendes EN, Queiroz DMM, Coimbra RS, et al. (1996) Experimental infection of Wistar rats with “Gastrospirillum suis”. J Med Microbiol 44:105–109

    PubMed  CAS  Google Scholar 

  22. Fox JG, Shen Z, Xu S, et al. (2002) Helicobacter marmotae sp. nov. Isolated from Livers of Woodchucks and Intestines of Cats. J Clin Microbiol 40:2513–2519

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This work was supported by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG), Brazil.

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Authors and Affiliations

  1. Department of Microbiology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, MG, CEP 31.270-901, Brazil

    Luíza B. Comunian, Sílvia B. Moura & Jacques R. Nicoli

  2. Faculdade de Ciências Biológicas, Centro Universitário Metodista Izabela Hendrix, Rua da Bahia 2020, Belo Horizonte, MG, CEP 30.160-012, Brazil

    Adriano P. Paglia

  3. Laboratory of Research in Bacteriology, Faculdade de Medicina, Universidade Federal de Minas Gerais, Av. Alfredo Balena 190, sala 4026, Belo Horizonte, MG, CEP 30130-100, Brazil

    Juliana B. Guerra, Gifone A. Rocha & Dulciene M. M. Queiroz

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  1. Luíza B. Comunian
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  2. Sílvia B. Moura
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  3. Adriano P. Paglia
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  4. Jacques R. Nicoli
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  5. Juliana B. Guerra
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  6. Gifone A. Rocha
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  7. Dulciene M. M. Queiroz
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Corresponding author

Correspondence to Sílvia B. Moura.

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Comunian, L.B., Moura, S.B., Paglia, A.P. et al. Detection of Helicobacter Species in the Gastrointestinal Tract of Wild Rodents From Brazil. Curr Microbiol 53, 370–373 (2006). https://doi.org/10.1007/s00284-005-0426-y

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  • DOI: https://doi.org/10.1007/s00284-005-0426-y

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