SNP-based next-generation sequencing reveals low-level mixed chimerism after allogeneic hematopoietic stem cell transplantation

  • Jieun Kim
  • In Sik Hwang
  • Saeam Shin
  • Jong Rak Choi
  • Seung-Tae Lee
Letter to the Editor



Hematopoietic stem cell transplantation


Short tandem repeat


Next-generation sequencing


Compliance with ethical standards

The study was approved by the Institutional Review Board (IRB) of Severance Hospital (approval no. 4-2016-0869) for the retrospective chart reviews and studies of existing preserved specimens all collected from subjects agreed and signed the informed consent.

Conflict of interest

The authors declare that they have no conflict of interest.


  1. 1.
    Barrett AJ, Battiwalla M (2010) Relapse after allogeneic stem cell transplantation. Expert Rev Hematol 3:429–441CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Khan F, Agarwal A, Agrawal S (2004) Significance of chimerism in hematopoietic stem cell transplantation: new variations on an old theme. Bone Marrow Transplant 34:1–12CrossRefPubMedGoogle Scholar
  3. 3.
    Clark JR, Scott SD, Jack AL, Lee H, Mason J, Carter GI, Pearce L, Jackson T, Clouston H, Sproul A, Keen L, Molloy K, Folarin N, Whitby L, Snowden JA, Reilly JT, Barnett D, United Kingdom National External Quality Assessment Service for Leucocyte Immunophenotyping Chimerism Working Group (2015) Monitoring of chimerism following allogeneic haematopoietic stem cell transplantation (HSCT): technical recommendations for the use of short tandem repeat (STR) based techniques, on behalf of the United Kingdom National External Quality Assessment Service for Leucocyte Immunophenotyping Chimerism Working Group. Br J Haematol 168:26–37CrossRefPubMedGoogle Scholar
  4. 4.
    Schuh A, Becq J, Humphray S, Alexa A, Burns A, Clifford R, Feller SM, Grocock R, Henderson S, Khrebtukova I, Kingsbury Z, Luo S, McBride D, Murray L, Menju T, Timbs A, Ross M, Taylor J, Bentley D (2012) Monitoring chronic lymphocytic leukemia progression by whole genome sequencing reveals heterogeneous clonal evolution patterns. Blood 120:4191–4196CrossRefPubMedGoogle Scholar
  5. 5.
    Nollet F, Billiet J, Selleslag D, Criel A (2001) Standardisation of multiplex fluorescent short tandem repeat analysis for chimerism testing. Bone Marrow Transplant 28:511–518CrossRefPubMedGoogle Scholar
  6. 6.
    Yang Y, Xie B, Yan J (2014) Application of next-generation sequencing technology in forensic science. Genomics Proteomics Bioinformatics 12:190–197CrossRefPubMedPubMedCentralGoogle Scholar
  7. 7.
    Roloff GW, Lai C, Hourigan CS, Dillon L (2017) Technical advances in the measurement of residual disease in acute myeloid leukemia. J Clin Med 6:87CrossRefPubMedCentralGoogle Scholar
  8. 8.
    Liesveld JL, Rothberg PG (2008) Mixed chimerism in SCT: conflict or peaceful coexistence? Bone Marrow Transplant 42:297–310CrossRefPubMedGoogle Scholar
  9. 9.
    Zhang S, Bian Y, Zhang Z et al (2015) Parallel analysis of 124 universal SNPs for human identification by targeted semiconductor sequencing. Sci Rep 5:18683CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jieun Kim
    • 1
  • In Sik Hwang
    • 2
  • Saeam Shin
    • 3
  • Jong Rak Choi
    • 4
  • Seung-Tae Lee
    • 4
  1. 1.Department of Laboratory MedicineSoonchunhyang University Seoul Hospital, Soonchunhyang University College of MedicineSeoulRepublic of Korea
  2. 2.Brain Korea 21 PLUS Project for Medical ScienceYonsei University College of MedicineSeoulRepublic of Korea
  3. 3.Department of Laboratory MedicineHallym University College of Medicine, Kangnam Sacred Heart HospitalSeoulRepublic of Korea
  4. 4.Department of Laboratory MedicineYonsei University College of MedicineSeoulRepublic of Korea

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