SNP-based next-generation sequencing reveals low-level mixed chimerism after allogeneic hematopoietic stem cell transplantation
Allogeneic hematopoietic stem cell transplantation (HSCT) is an important treatment modality for various malignant and non-malignant hematological disorders. Although complete donor-derived hematopoiesis, i.e., complete chimerism, is a desirable outcome, relapse of the original malignancy after HSCT remains the most frequent cause of treatment failure and mortality . Therefore, detection of the presence of recipient cellular components, referred to as chimerism monitoring, is critical for the prediction of clinical outcomes.
The detection of chimerism mainly employs molecular methods used in forensic sciences such as flow cytometry, cytogenetic analysis, fluorescence in situ hybridization, and short tandem repeat (STR)-based techniques; however, these have limited applicability and low detection sensitivity (in the range of 3–5%) . At present, STR analysis is the most popular method for monitoring chimerism following allogeneic HSCT; however, this method suffers...
Hematopoietic stem cell transplantation
Short tandem repeat
This study was supported by a grant of the National Research Foundation of Korea (NRF-2015R1D1A1A01059210).
Compliance with ethical standards
The study was approved by the Institutional Review Board (IRB) of Severance Hospital (approval no. 4-2016-0869) for the retrospective chart reviews and studies of existing preserved specimens all collected from subjects agreed and signed the informed consent.
Conflict of interest
The authors declare that they have no conflict of interest.
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