Irrigation Science

, Volume 36, Issue 1, pp 49–60 | Cite as

Regulated deficit irrigation effects on physiological parameters, yield, fruit quality and antioxidants of Vaccinium corymbosum plants cv. Brigitta

  • Tomás E. Lobos
  • Jorge B. Retamales
  • Samuel Ortega-Farías
  • Eric J. Hanson
  • Rafael López-Olivari
  • María L. Mora
Original Paper


Highbush blueberries require sufficient water throughout the growing season, especially during fruit development. Regulated deficit irrigation (RDI) has been used in many fruit crops to save water and potentially increase fruit quality without reducing yield. Our aim was to determine the effects of RDI on yield, plant–water relations, and fruit quality at harvest. Three irrigation treatments based on actual evapotranspiration (ETa) were applied: 50, 75 and 100% (control) to six-year-old ‘Brigitta’ highbush blueberries in Colbún, Maule Region, Chile, for seasons 2013–2014 and 2014–2015 and to 26-year-old ‘Brigitta’ plants in South Haven, Michigan, USA, during the 2014 season. Severe water deficit treatment (50% ETa) decreased photosynthetic rate, vegetative growth (second season in Colbún and South Haven’s trial), and fruit quality (berry size, titratable acidity, soluble solids, weight), and increased oxidative stress during both seasons in Colbún. The 50% ETa treatment also had the highest yield reduction during Colbún’s second season. In contrast, mild water stress (75% ETa) resulted in similar fruit yields and quality (firmness, fruit size, titratable acidity, soluble solids and berry weight) but with higher water productivity than the 100% ETa treatment, while it achieved intermediate values for antioxidant capacity compared to the 50% ETa treatment.


Abiotic stress Blueberry Water management Water stress Plant performance Plant growth 



The authors would like to thank: Doctorado en Ciencias de Recursos Naturales; CONICYT scholarship 21110856 and 2968/2015; Fulbright and Becas-Chile visiting research scholar grant (2714/2011); Universidad de La Frontera; Universidad de Talca, Research program on Adaptation of Agriculture to Climate Change (PIEI A2C2); CITRA-U. Talca fellowship; Michigan State University; Agrícola Sofama and DeGrandchamps farms.


  1. Acevedo-Opazo C, Ortega-Farias S, Fuentes S (2010) Effects of grapevine (Vitis vinifera L.) water status on water consumption, vegetative growth and grape quality: an irrigation scheduling application to achieve regulated deficit irrigation. Agric Water Manage 97:956–964. CrossRefGoogle Scholar
  2. Améglio T, Le Roux X, Mingeau M, Perrier C (2000) Water relations of highbush blueberry under drought conditions. Acta Hortic 537:273–278. CrossRefGoogle Scholar
  3. Bacelar EA, Santos DL, Moutinho-Pereira JM et al (2007) Physiological behaviour, oxidative damage and antioxidative protection of olive trees grown under different irrigation regimes. Plant Soil 292:1–12. CrossRefGoogle Scholar
  4. Boland AM, Jerie PH, Mitchell PD et al (2000) Long-term effects of restricted root volume and regulated deficit irrigation on peach: II. Productivity and water use. J Am Soc Hortic Sci 125:143–148Google Scholar
  5. Bordonaba JG, Terry LA (2010) Manipulating the taste-related composition of strawberry fruits (Fragaria × ananassa) from different cultivars using deficit irrigation. Food Chem 122:1020–1026. CrossRefGoogle Scholar
  6. Brauman K a, Siebert S, Foley J a (2013) Improvements in crop water productivity increase water sustainability and food security—a global analysis. Environ Res Lett 8:24030. CrossRefGoogle Scholar
  7. Bryla DR (2008) Water requirements of young blueberry plants irrigated by sprinklers, microsprays and drip. Acta Hortic 135–139.
  8. Bryla D, Strik BC (2006) Variation in plant and soil water relations among irrigated blueberry cultivars planted at two distinct in-row spacings. Acta Hortic 715:295–300. CrossRefGoogle Scholar
  9. Bryla DR, Strik BC (2007) Effects of cultivar and plant spacing on the seasonal water requirements of highbush blueberry. J Am Soc Hortic Sci 132:270–277Google Scholar
  10. Bryla DR, Yorgey B, Shireman AD (2009) Irrigation management effects on yield and fruit quality of highbush blueberry. Acta Hortic 649–656.
  11. Bryla D, Gartung J, Strik B (2011) Evaluation of irrigation methods for highbush blueberry—I. Growth and water requirements of young plants. HortScience 46:95–101Google Scholar
  12. Castrejón ADR, Eichholz I, Rohn S et al (2008) Phenolic profile and antioxidant activity of highbush blueberry (Vaccinium corymbosum L.) during fruit maturation and ripening. Food Chem 109:564–572. CrossRefGoogle Scholar
  13. Choné X, Van Leeuwen C, Dubourdie D, Gaudillères JP (2001) Stem water potential is a sensitive indicator of grapevine water status. Ann Bot (Lond) 87:477–483. CrossRefGoogle Scholar
  14. Concha-Meyer A, Eifert JD, Williams RC et al (2015) Shelf life determination of fresh blueberries (Vaccinium corymbosum) stored under controlled atmosphere and ozone. Int J Food Sci. PubMedPubMedCentralGoogle Scholar
  15. Connor AM, Luby JJ, Tong CBS et al (2002) Genotypic and environmental variation in antioxidant activity, total phenolic content, and anthocyanin content among blueberry cultivars. J Am Soc Hortic Sci 127:89–97Google Scholar
  16. Cruz de Carvalho MH (2008) Drought stress and reactive oxygen species. Plant Signal Behav 3:156–165. CrossRefPubMedPubMedCentralGoogle Scholar
  17. Cui N, Du T, Kang S et al (2008) Regulated deficit irrigation improved fruit quality and water use efficiency of pear-jujube trees. Agric Water Manage 95:489–497. CrossRefGoogle Scholar
  18. Du Z, Bramlage WJ (1992) Modified thiobarbituric acid assay for measuring lipid oxidation in sugar-rich plant tissue extracts. J Agric Food Chem 40:1566–1570. CrossRefGoogle Scholar
  19. Ehlenfeldt MK, Martin RB (2002) A survey of fruit firmness in highbush blueberry and species-introgressed blueberry cultivars. HortScience 37:386–389Google Scholar
  20. Ehret DL, Frey B, Forge T et al (2012) Effects of drip irrigation configuration and rate on yield and fruit quality of young Highbush blueberry plants. HortScience 47:414–421Google Scholar
  21. Ehret DL, Frey B, Forge T et al (2015) Age-related changes in response of highbush blueberry plants to drip irrigation. HortScience 50:486–490Google Scholar
  22. Estrada F, Escobar A, Romero-Bravo S et al (2015) Fluorescence phenotyping in blueberry breeding for genotype selection under drought conditions, with or without heat stress. Sci Hortic 181:147–161. CrossRefGoogle Scholar
  23. Fereres E, Soriano MA (2007) Deficit irrigation for reducing agricultural water use. J Exp Bot 58:147–159. CrossRefPubMedGoogle Scholar
  24. Flexas J, Barón M, Bota J et al (2009) Photosynthesis limitations during water stress acclimation and recovery in the drought-adapted Vitis hybrid Richter-110 (V. berlandierixV. rupestris). J Exp Bot 60:2361–2377. CrossRefPubMedGoogle Scholar
  25. García-Tejero I, Jiménez-Bocanegra JA, Martínez G et al (2010) Positive impact of regulated deficit irrigation on yield and fruit quality in a commercial citrus orchard [Citrus sinensis (L.) Osbeck, cv. salustiano]. Agric Water Manage 97:614–622. CrossRefGoogle Scholar
  26. Giovanelli G, Buratti S (2009) Comparison of polyphenolic composition and antioxidant activity of wild Italian blueberries and some cultivated varieties. Food Chem 112:903–908. CrossRefGoogle Scholar
  27. Goldhamer DA, Viveros M, Salinas M (2006) Regulated deficit irrigation in almonds: Effects of variations in applied water and stress timing on yield and yield components. Irrig Sci 24:101–114. CrossRefGoogle Scholar
  28. Grant OM (2012) Understanding and exploiting the impact of drought stress on plant physiology. In: Ahmad P, Prasad MNV (eds) Abiotic stress responses in plants. Springer New York, New York, pp 89–104CrossRefGoogle Scholar
  29. Guo Z, Ou W, Lu S, Zhong Q (2006) Differential responses of antioxidative system to chilling and drought in four rice cultivars differing in sensitivity. Plant Physiol Biochem 44:828–836. CrossRefPubMedGoogle Scholar
  30. Holzapfel E, Hepp R, Mariño M (2004) Effect of irrigation on fruit production in blueberry. Agric Water Manage 67:173–184. CrossRefGoogle Scholar
  31. Holzapfel E, Jara J, Coronata AM (2015) Number of drip laterals and irrigation frequency on yield and exportable fruit size of highbush blueberry grown in a sandy soil. Agric Water Manage 148:207–212. CrossRefGoogle Scholar
  32. Howell TA (2001) Enhancing water use efficiency in irrigated agriculture. Agron J 93:281–289. CrossRefGoogle Scholar
  33. Intrigliolo DS, Castel JR (2010) Response of grapevine cv. “Tempranillo” to timing and amount of irrigation: water relations, vine growth, yield and berry and wine composition. Irrig Sci 28:113–125. CrossRefGoogle Scholar
  34. Iturbe-Ormaetxe I (1998) Oxidative damage in pea plants exposed to water deficit or paraquat. Plant Physiol 116:173–181. CrossRefPubMedCentralGoogle Scholar
  35. Jara-Rojas F, Ortega-Farías S, Valdés-Gómez H, Acevedo-Opazo C (2015) Gas exchange relations of ungrafted grapevines (cv. Carménère) growing under irrigated field conditions. S Afr J Enol Vitic 36:231–242Google Scholar
  36. Johnson HE, Broadhurst D, Goodacre R, Smith AR (2003) Metabolic fingerprinting of salt-stressed tomatoes. Phytochemistry 62:919–928. CrossRefPubMedGoogle Scholar
  37. Jorquera-Fontena E, Alberdi M, Franck N (2014) Pruning severity affects yield, fruit load and fruit and leaf traits of “Brigitta” blueberry. J Soil Sci Plant Nutr 14:855–868Google Scholar
  38. Kalt W, Lawand C (2003) Oxygen radical absorbing capacity, anthocyanin and phenolic content of highbush blueberries (Vaccinium corymbosum L.) during ripening and storage. J Am Hortic Sci 128:917–923Google Scholar
  39. Keen B, Slavich P (2012) Comparison of irrigation scheduling strategies for achieving water use efficiency in highbush blueberry. N Zeal J Crop Hortic Sci 40:3–20. CrossRefGoogle Scholar
  40. Lei S, Yunzhou Q, Fengchao J et al (2009) Physiological mechanism contributing to efficient use of water in field tomato under different irrigation. Plant Spoil Environ 55:128–133Google Scholar
  41. Liu MC, Kojima T, Tanaka M, Chen H (2001) Effect of soil moisture on plant growth and fruit properties of strawberry. Acta Horticulturae Sinica 28:307–311Google Scholar
  42. Lobos TE, Retamales JB, Ortega-Farías S et al (2016) Pre-harvest regulated deficit irrigation management effects on post-harvest quality and condition of V. corymbosum fruits cv. Brigitta Scientia Horticulturae 207:152–159. CrossRefGoogle Scholar
  43. Lohachoompol V, Srzednicki G, Craske J (2004) The change of total anthocyanins in blueberries and their antioxidant effect after drying and freezing. J Biomed Biotechnol 2004:248–252. CrossRefPubMedPubMedCentralGoogle Scholar
  44. Miller G, Suzuki N, Ciftci-Yilmaz S, Mittler R (2010) Reactive oxygen species homeostasis and signalling during drought and salinity stresses. Plant Cell Environ 33:453–467. CrossRefPubMedGoogle Scholar
  45. Mingeau M, Perrier C, Améglio T (2001) Evidence of drought-sensitive periods from flowering to maturity on highbush blueberry. Sci Hortic 89:23–40. CrossRefGoogle Scholar
  46. Morales CG, Pino MT, del Pozo A (2013) Phenological and physiological responses to drought stress and subsequent rehydration cycles in two raspberry cultivars. Sci Hortic 162:234–241. CrossRefGoogle Scholar
  47. Mpelasoka BS, Behboudian MH, Green SR (2001) Water use, yield and fruit quality of lysimeter-grown apple trees: responses to deficit irrigation and to crop load. Irrig Sci 20:107–113. CrossRefGoogle Scholar
  48. Ortega-Farias S, Fereres E, Sadras VO (2012) Special issue on water management in grapevines. Irrig Sci 30:335–337. CrossRefGoogle Scholar
  49. Paniagua ACC, East ARR, Hindmarsh JPP, Heyes JA a (2013) Moisture loss is the major cause of firmness change during postharvest storage of blueberry. Postharvest Biol Technol 79:13–19. CrossRefGoogle Scholar
  50. Perrier C, Mingeau M, Améglio T (2000) Effects of water stress on transpiration, radial growth and yield in highbush blueberry. Acta Hortic 923–928.
  51. Prange RK, DeEll JR (1997) Preharvest factors affecting postharvest quality of berry crops. HortScience 32:824–830Google Scholar
  52. Prior RL, Hoang H, Gu L et al (2003) Assays for hydrophilic and lipophilic antioxidant capacity (oxygen radical absorbance capacity (ORAC FL)) of plasma and other biological and food samples. J Agric Food Chem 51:3273–3279. CrossRefPubMedGoogle Scholar
  53. Retamales JB, Hancock JF (2012a) Blueberry field management and harvesting. In: Blueberries, 1st edn. CABI, Cambridge, p 323Google Scholar
  54. Retamales JB, Hancock JF (2012b) Pre- and postharvest management of fruit quality. In: Blueberries, 1st edn. CABI, Cambridge, p 323Google Scholar
  55. Retamales JB, Hanson EJ, Bukovac MJ (2000) GA3 as a flowering inhibitor in blueberries. Acta Hortic 147–152.
  56. Retamales JB, Mena C, Lobos G, Morales Y (2015) A regression analysis on factors affecting yield of highbush blueberries. Sci Hortic 186:7–14. CrossRefGoogle Scholar
  57. Reyes-Díaz M, Meriño-Gergichevich C, Alarcón E et al (2011) Calcium sulfate ameliorates the effect of aluminum toxicity differentially in genotypes of highbush blueberry (Vaccinium corymbosum L.). J Soil Sci Plant Nutr 11:59–78. CrossRefGoogle Scholar
  58. Rho H, Yu DJ, Kim SJ, Lee HJ (2012) Limitation factors for photosynthesis in “Bluecrop” highbush blueberry (Vaccinium corymbosum) leaves in response to moderate water stress. J Plant Biol 55:450–457. CrossRefGoogle Scholar
  59. Ribera AE, Reyes-Diaz M, Alberdi M et al (2010) Antioxidant compounds in skin and pulp of fruits change among genotypes and maturity stages in highbush blueberry (Vaccinium corymbosum L.) grown in southern Chile. J Soil Sci Plant Nutr 10:509–536. CrossRefGoogle Scholar
  60. Ruiz Sánchez MC, Domingo Miguel R, Castel Sánchez JR et al (2010) Deficit irrigation in fruit trees and vines in Spain. Span J Agric Res 8:5–20. CrossRefGoogle Scholar
  61. Sams CE (1999) Preharvest factors affecting postharvest texture. Postharvest Biol Technol 15:249–254. CrossRefGoogle Scholar
  62. Santesteban LG, Miranda C, Royo JB (2011) Regulated deficit irrigation effects on growth, yield, grape quality and individual anthocyanin composition in Vitis vinifera. L. cv. “Tempranillo”. Agric Water Manag 98:1171–1179. CrossRefGoogle Scholar
  63. Strik B, Buller G, Hellman E (2003) Pruning severity affects yield, berry weight, and hand harvest efficiency of highbush blueberry. HortScience 38:196–199Google Scholar
  64. Szőllősi R (2014) Superoxide dismutase (SOD) and abiotic stress tolerance in plants. In: Ahmad P (ed) Oxidative damage to plants. Elsevier, New York, pp 89–129Google Scholar
  65. Zarrouk O, Francisco R, Pinto-Marijuan M et al (2012) Impact of irrigation regime on berry development and flavonoids composition in Aragonez (Syn. Tempranillo) grapevine. Agric Water Manage 114:18–29. CrossRefGoogle Scholar
  66. Zheng Y, Wang CY, Wang SY, Zheng W (2003) Effect of high-oxygen atmospheres on blueberry phenolics, anthocyanins, and antioxidant capacity. J Agric Food Chem 51:7162–7169. CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Tomás E. Lobos
    • 2
  • Jorge B. Retamales
    • 3
  • Samuel Ortega-Farías
    • 4
  • Eric J. Hanson
    • 5
  • Rafael López-Olivari
    • 6
  • María L. Mora
    • 1
  1. 1.Center of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource NucleusUniversidad de La FronteraTemucoChile
  2. 2.Programa de Doctorado en Ciencias de Recursos NaturalesUniversidad de La FronteraTemucoChile
  3. 3.Departamento de Horticultura, Facultad de Ciencias AgrariasUniversidad de TalcaTalcaChile
  4. 4.Centro de Investigación y Transferencia en Riego y Agroclimatología (CITRA) and Research program on Adaptation of Agriculture to Climate Change (PIEI A2C2)Universidad de TalcaTalcaChile
  5. 5.Department of Horticulture, A338 Plant and Soil Science Bldg.Michigan State UniversityEast LansingUSA
  6. 6.Centro Regional de Investigaciones CarillancaInstituto de Investigaciones Agropecuarias TemucoChile

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