International Orthopaedics

, Volume 42, Issue 4, pp 939–946 | Cite as

Effects of continuous or intermittent low-magnitude high-frequency vibration on fracture healing in sheep

  • Yanhui Li
  • Guozhao Liu
  • Jing Yu
  • Chen Li
  • Lei Tan
  • Baohui Hao
  • Chao Liu
  • Junhao Lin
  • Dong Zhu
  • Xizheng Zhang
Original Paper



Vibration therapy has been shown to improve fracture healing. In this study, we investigated the effects of continuous or different intermittent vibration regimens on fracture healing in sheep models on the basis of radiographs, mechanical, and biochemical testing.


The 63 right-hind metatarsals from 63 sheep (12-month-old) were osteotomized; followed by surgical fixation with a steel plate. Two weeks after the surgery, the sheep with right-hind metatarsal fractures were randomly divided into seven groups (n=9/group): control (no vibration treated), continuous vibration (CV), one, three, five, seven and 14-day intermittent vibration (named IV-1, -3, -5, -7, and -14, respectively) groups, which represented a cycle of the successive n-day vibration and successive n-day break. Vibration stimulation (F=35 Hz, a=0.25 g) lasted 15 minutes each treatment. After eight weeks with/without vibration treatment, the sheep were euthanized with intravenous anesthetic. The callus formation, mechanical properties, and biochemical compositions of fracture metatarsals were analyzed.


In CV and IV-7 groups, X-ray images showed an increased callus volume around the fracture area. The bone elastic modulus and the concentrations of Ca, P, and Ca/P ratio of the area at 15 and 25 mm away from the fracture centerline were higher in CV and IV-7 groups compared with the other groups.


Our results demonstrate that both CV and IV-7 vibration patterns showed better improvement of fracture healing.


Continuous or intermittent vibration Fracture healing X-ray imaging Mechanical and biochemical properties 



This work was supported by the Program of the National Natural Science Foundation of China (Nos. 11432016) and the Program of the National Natural Science Foundation of China (Nos. 11602093).

Compliance with ethical standards

Animals used in this article were approved by the Experimental Animal Management Guide of University of Jilin (Changchun, China).

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

264_2018_3759_MOESM1_ESM.docx (60 kb)
ESM 1 (DOCX 59 kb)


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Copyright information

© SICOT aisbl 2018

Authors and Affiliations

  • Yanhui Li
    • 1
  • Guozhao Liu
    • 2
  • Jing Yu
    • 3
  • Chen Li
    • 4
  • Lei Tan
    • 4
  • Baohui Hao
    • 4
  • Chao Liu
    • 4
  • Junhao Lin
    • 2
  • Dong Zhu
    • 4
  • Xizheng Zhang
    • 5
  1. 1.Department of Cardiology and EchocardiographyFirst Hospital of Jilin UniversityChangchunChina
  2. 2.College of Mechanical Science and EngineeringJilin UniversityChangchunChina
  3. 3.Operating Theatre 1The First Hospital of Jilin UniversityChangchunChina
  4. 4.Department of Orthopedic TraumatologyFirst Hospital of Jilin UniversityChangchunChina
  5. 5.Institute of Medical EquipmentAcademy of Military Medical SciencesTianjinChina

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