Programmed cell death ligand-1 (PD-L1) and cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) play a pivotal role in cancer immunotherapy. Each of these molecules has a membrane-bound receptor form (mPD-L1/mCTLA-4) and a soluble form (sPD-L1/sCTLA-4). However, these prognostic impacts in colorectal cancer (CRC) remain unclear.
We immunohistochemically scored tumoral mPD-L1/mCTLA-4 expression and quantified preoperative circulating sPD-L1/sCTLA-4 levels using matched serum specimens from 131 patients with pStage I–III CRC. We also examined the association between these statuses and tumor infiltrating lymphocytes (TILs) in these patients.
Elevated levels of mPD-L1, mCTLA-4, sPD-L1 and sCTLA-4 were significantly correlated with poor overall survival (OS) and disease-free survival (DFS). Co-high expression of tumoral mPD-L1 and mCTLA-4 or co-elevated levels of serum sPD-L1 and sCTLA-4 were strongly correlated with poor OS and DFS. Multivariate analysis revealed that both statuses were negative independent prognostic factors for OS [hazard ratio (HR) 3.86, 95% confidence interval (95% CI) 1.71–8.51, p = 0.001; HR 5.72, 95% CI 1.87–14.54, p = 0.004, respectively] and DFS (HR 2.53, 95% CI 1.23–4.95, p = 0.01; HR 6.88, 95% CI 2.42–17.13, p = 0.0008, respectively). Although low expression of tumoral mCTLA-4 was significantly correlated with increased CD8(+) TILs, there was no correlation in any other combination.
We verified the prognostic impacts of mPD-L1, mCTLA-4, sPD-L1 and sCTLA-4 in pStage I–III CRC patients. Dual evaluation of immune checkpoint molecules in primary tissues or preoperative serum could identify a patient population with poor prognosis in these patients.
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Cytotoxic T-lymphocyte-associated antigen 4
Programmed cell death-1
Programmed cell death ligand-1
Receiver operating characteristic
- Treg cells:
Regulatory T cells
Tumor infiltrating lymphocytes
- 95% CI:
95% confidence interval
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We thank all the patients, their families, and the investigators involved in this study. We also thank Mrs. Yuki Orito and Mrs. Amphone Okada for their excellent technical assistance and R J Frampton from Edanz Group for editing a draft of this manuscript.
The work was partially supported by a Grant in Aid for Scientific Research (16K10533, 18K08592) from the Ministry of Education, Culture, Sports, Science, and Technology, Japan.
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The authors have no conflicts of interest to disclose.
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Supplementary Figure 3. Receiver operating characteristic (ROC) curve analysis for overall survival to decide the cut-off value of tumoral and circulating expression of immune checkpoints. (a) mPD-L1 (sensitivity: 0.92, specificity: 0.28, AUC: 0.57, cut-off: 4 (score of expression)) (b) mCTLA-4 (sensitivity: 0.48, specificity: 0.86, AUC: 0.65, cut-off: 6 (score of expression)) (c) sPD-L1 (sensitivity: 0.40, specificity: 0.86, AUC: 0.59, cut-off: 0.08 (ng/ml)) (d) sCTLA-4 (sensitivity: 0.32, specificity: 0.87, AUC: 0.51, cut-off: 1.79 (ng/ml)) (PDF 144 kb)
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Omura, Y., Toiyama, Y., Okugawa, Y. et al. Prognostic impacts of tumoral expression and serum levels of PD-L1 and CTLA-4 in colorectal cancer patients. Cancer Immunol Immunother (2020). https://doi.org/10.1007/s00262-020-02645-1
- Tumoral membrane expression
- Soluble receptors and ligands
- Colorectal Cancer