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Bacterial tannases: classification and biochemical properties

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Abstract

Tannin acyl hydrolases, also known as tannases, are a group of enzymes critical for the transformation of tannins. The study of these enzymes, which initially evolved in different organisms to detoxify and/or use these plant metabolites, has nowadays become relevant in microbial enzymology research due to their relevant role in food tannin transformation. Microorganisms, particularly bacteria, are major sources of tannase. Cloning and heterologous expression of bacterial tannase genes and structural studies have been performed in the last few years. However, a systematic compilation of the information related to all recombinant tannases, their classification, and characteristics is missing. In this review, we explore the diversity of heterologously produced bacterial tannases, describing their substrate specificity and biochemical characterization. Moreover, a new classification based on sequence similarity analysis is proposed. Finally, putative tannases have been identified in silico for each group of tannases taking advantage of the use of the “tannase” distinctive features previously proposed.

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Acknowledgements

We thank MINEICO for the Severo Ochoa Excellence accreditation (SEV-2016-0644).

Funding

This work was financially supported by grants AGL2014-52911-R and SAF2015-73549-JIN from the Spanish Ministry of Economy and Competitiveness/FEDR-MINEICO.

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Authors and Affiliations

  1. Laboratorio de Biotecnología Bacteriana, Instituto de Ciencia y Tecnología de Alimentos y Nutrición, ICTAN-CSIC, Juan de la Cierva 3, 28006, Madrid, Spain

    Blanca de las Rivas & Rosario Muñoz

  2. Macrophage and Tick Vaccine Laboratory, CIC bioGUNE, Derio, Biscay, Spain

    Héctor Rodríguez & Juan Anguita

  3. Ikerbasque, Basque Foundation for Science, Bilbao, Biscay, Spain

    Juan Anguita

Authors
  1. Blanca de las Rivas
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  2. Héctor Rodríguez
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  3. Juan Anguita
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  4. Rosario Muñoz
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Correspondence to Rosario Muñoz.

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de las Rivas, B., Rodríguez, H., Anguita, J. et al. Bacterial tannases: classification and biochemical properties. Appl Microbiol Biotechnol 103, 603–623 (2019). https://doi.org/10.1007/s00253-018-9519-y

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  • DOI: https://doi.org/10.1007/s00253-018-9519-y

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