Abstract
Inorganic polyphosphate (polyP) is a significant regulatory and metabolic compound in yeast cells. We compared polyP content and localization, polyphosphatase activities, and transcriptional profile of polyP-related genes in industrially important methylotrophic yeasts, Hansenula polymorpha and Pichia pastoris. The increased need for phosphate, the decrease of long-chain polyP level, the accumulation of short-chain polyP, and enhanced endopolyphosphatase activity in the crude membrane fraction were observed in methanol-grown cells compared with glucose-grown cells of both species. Transcriptome analysis revealed notable differences in the expression patterns of key genes encoding proteins related to polyP metabolism. In methanol-grown cells, the genes encoding endopolyphosphatases and phosphate transporters were upregulated. The changes in polyP metabolism are probably related to the peculiarities of bioenergetics of methanol-grown cells.
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The authors thank Elena Makeeva for her help with preparing the manuscript.
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This study was funded by the Russian Foundation for Basic Research (Grant No. 17-04-00822).
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Andreeva, N., Ryazanova, L., Zvonarev, A. et al. Inorganic polyphosphate in methylotrophic yeasts. Appl Microbiol Biotechnol 102, 5235–5244 (2018). https://doi.org/10.1007/s00253-018-9008-3
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DOI: https://doi.org/10.1007/s00253-018-9008-3