Seeking key microorganisms for enhancing methane production in anaerobic digestion of waste sewage sludge
- 213 Downloads
Efficient approaches for the utilization of waste sewage sludge have been widely studied. One of them is to use it for the bioenergy production, specifically methane gas which is well-known to be driven by complex bacterial interactions during the anaerobic digestion process. Therefore, it is important to understand not only microorganisms for producing methane but also those for controlling or regulating the process. In this study, azithromycin analogs belonging to macrolide, ketolide, and lincosamide groups were applied to investigate the mechanisms and dynamics of bacterial community in waste sewage sludge for methane production. The stages of anaerobic digestion process were evaluated by measuring the production of intermediate substrates, such as protease activity, organic acids, the quantification of bacteria and archaea, and its community dynamics. All azithromycin analogs used in this study achieved a high methane production compared to the control sample without any antibiotic due to the efficient hydrolysis process and the presence of important fermentative bacteria and archaea responsible in the methanogenesis stage. The key microorganisms contributing to the methane production may be Clostridia, Cladilinea, Planctomycetes, and Alphaproteobacteria as an accelerator whereas Nitrosomonadaceae and Nitrospiraceae may be suppressors for methane production. In conclusion, the utilization of antibiotic analogs of macrolide, ketolide, and lincosamide groups has a promising ability in finding the essential microorganisms and improving the methane production using waste sewage sludge.
KeywordsMacrolide Ketolide Lincosamide Sewage sludge Methane Microbial community
The authors wish to thank the Japanese Government Scholarship (MEXT), Kitakyushu City, and Science & Technology Research Partnership for Sustainable Development Program (SATREPS) for the support of this study.
Compliance with ethical standards
This article does not contain any studies performed with human participants or with animals by any of the authors.
Conflict of interest
The authors declare that there are no conflicts of interest.
- Chen Q (2010) Kinetics of anaerobic digestion of selected C1 to C4 organic acids. Dissertation, University of Missouri—ColumbiaGoogle Scholar
- Elshahed MS, Youssef NH, Luo Q, Najar FZ, Roe BA, Sisk TM, Bühring SI, Hinrichs KU, Krumholz LR (2007) Phylogenetic and metabolic diversity of Planctomycetes from anaerobic, sulfide- and sulfur-rich Zodletone Spring, Oklahoma. Appl Environ Microbiol 73:4707–4716CrossRefPubMedPubMedCentralGoogle Scholar
- Ettwig KF, Speth DR, Reimann J, Wu ML, Jetten MS, Keltjens JT (2012) Bacterial oxygen production in the dark. Front Microbiol 3:273Google Scholar
- Fan C, Lee PKH, Ng WJ, Alvarez-Cohen L, Brodie EL, Andersen GL, He J (2009) Influence of trace erythromycin and erythromycin-H2O on carbon and nutrients removal and on resistance selection in sequencing batch reactors (SBRs). Appl Microbiol Biotechnol 85:185–195CrossRefPubMedPubMedCentralGoogle Scholar
- Goldstein EJC, Citron DM, Merriam CV, Warren Y, Tyrrel KL, Fernandez H (2003) In vitro activities of telithromycin and 10 oral agents against aerobic and anaerobic pathogens isolated from antral puncture specimens from patients with sinusitis. Antimicrob Agents Chemother 47:1963–1967CrossRefPubMedPubMedCentralGoogle Scholar
- Hammer Ø, Harper DAT, Ryan PD (2001) Paleontological statistics software: package for education and data analysis. Palaeontol Electron 4Google Scholar
- Liu PY, Chen JR, Shao L, Tan J, Chen DJ (2018) Responses of flocculent and granular sludge in anaerobic sequencing batch reactor (ASBR) to azithromycin wastewater and its impact on microbial communities. J Chem Technol Biotechnol. https://doi.org/10.1002/jctb.5578
- Manyi-Loh CE, Mamphweli SN, Meyer EL, Okoh AI, Makaka G, Simon M (2013) Microbial anaerobic digestion (bio-digesters) as an approach to the decontamination of animal wastes in pollution control and the generation of renewable energy. Int J Environ Res Public Health 10:4390–4417CrossRefPubMedPubMedCentralGoogle Scholar
- Senta I, Krizman-Matasic I, Terzic S, Ahel M (2017) Comprehensive determination of macrolide antibiotics, their synthesis intermediates and transformation products in wastewater effluents and ambient waters by liquid chromatography–tandem mass spectrometry. J Chromatogr A 1509:60–68CrossRefPubMedGoogle Scholar
- Smieja M (1998) Current indications for the use of clindamycin: a critical review. Can J Infect Dis Med Microbiol 9:22–28Google Scholar
- Terzic S, Udikovic-Kolic N, Jurina T, Krizman-Matasic I, Senta I, Mihaljevic I, Loncar J, Smital T, Ahel M (2018) Biotransformation of macrolide antibiotics using enriched activated sludge culture: kinetics, transformation routes and ecotoxicological evaluation. J Hazard Mater 349:143–152CrossRefPubMedGoogle Scholar