Applied Microbiology and Biotechnology

, Volume 102, Issue 12, pp 5165–5172 | Cite as

A novel agaro-oligosaccharide-lytic β-galactosidase from Agarivorans gilvus WH0801

  • Xiaoqing Yang
  • Zhen Liu
  • Chengcheng Jiang
  • Jianan Sun
  • Changhu Xue
  • Xiangzhao Mao
Biotechnologically relevant enzymes and proteins
  • 123 Downloads

Abstract

β-Galactosidases have a wide application in the food and pharmaceutical industries. Recently, β-galactosidase was also found to participate in agar degradation. In this study, the second reported agarolytic β-galactosidase was found in the marine bacterium Agarivorans gilvus WH0801 and characterized. The β-galactosidase named AgWH2A (83 kDa) exhibited good activities under optimal hydrolysis conditions of pH 8.0 and 40 °C. AgWH2A could cleave the first D-galactose of agarooligosaccharides from its nonreducing end to produce neoagarooligosaccharides, but could not act on the neoagarooligosaccharides. AgWH2A has great potential in the comprehensive utilization of marine red algae.

Keywords

β-Galactosidase GH2 family Agarivorans gilvus D-Galactose Agarooligosaccharides 

Notes

Funding information

This work was supported by the National Natural Science Foundation of China (31471607) and the Applied Basic Research Program of Qingdao (16-5-1-17-jch).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Food Science and EngineeringOcean University of ChinaQingdaoChina
  2. 2.Laboratory for Marine Drugs and BioproductsQingdao National Laboratory for Marine Science and TechnologyQingdaoChina

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