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Monitoring the impact of an aspartic protease (MpAPr1) on grape proteins and wine properties

  • Biotechnologically relevant enzymes and proteins
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Abstract

The perception of haze in wine is brought about when pathogenesis-related proteins become unstable and aggregate, subsequently resulting in crosslinking until it develops into light-dispersing particles. Elimination of these proteins is usually achieved via bentonite fining, which, although effective, suffers from several drawbacks. The utilization of proteases has been proposed as an ideal alternative. In a previous study, an aspartic protease (MpAPr1) from the yeast Metschnikowia pulcherrima was purified and shown to be partially active against grape proteins in synthetic medium. In this study, the effects of pure MpAPr1 supplemented to Sauvignon Blanc juice on subsequent fermentation were investigated. The juice was incubated for 48 h and thereafter inoculated with Saccharomyces cerevisiae. Results revealed that the enzyme had no observable effects on fermentation performance and retained activity throughout. Protein degradation could be detected and resulted in a significant modification of the wine composition and an increase in the presence of certain volatile compounds, especially those linked to amino acid metabolism.

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Acknowledgements

The authors thank the Central Analytical Facility, Stellenbosch University, for their technical support.

Funding

The author would like to thank the National Research Foundation of South Africa (UID: 88819) and Winetech for their financial support.

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Authors and Affiliations

  1. Institute for Wine Biotechnology, Department of Viticulture and Oenology, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa

    Louwrens Wiid Theron & Benoit Divol

  2. Univ. Bordeaux, Unité de Recherche Œnologie, EA 4577, USC 1366 INRA, ISVV, 33882, Villenave d’Ornon Cedex, France

    Louwrens Wiid Theron & Marina Bely

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  1. Louwrens Wiid Theron
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  2. Marina Bely
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Correspondence to Benoit Divol.

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Theron, L.W., Bely, M. & Divol, B. Monitoring the impact of an aspartic protease (MpAPr1) on grape proteins and wine properties. Appl Microbiol Biotechnol 102, 5173–5183 (2018). https://doi.org/10.1007/s00253-018-8980-y

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  • DOI: https://doi.org/10.1007/s00253-018-8980-y

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