High diversity of potential nitrate-reducing Fe(II)-oxidizing bacteria enriched from activated sludge
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Nitrate-dependent Fe(II) oxidation (NDFO) has been discovered in various environments including activated sludge and can potentially be used to remove nitrate from wastewater. In this study, NDFO sludge was successfully enriched from activated sludge under high Fe(II) concentrations over 100 days and the denitrification rate achieved 1.37 mmol N/(gVSS day). High-throughput sequencing of the bacterial 16S rRNA gene was used to investigate the microbial community structure dynamics during the enrichment process. The results showed that the microbial community changed significantly and high diversity of potential Fe(II)-oxidizing bacteria (FeOB) was observed in the enriched sludge. Thermomonas and Gallionella were the dominant bacterial genera in the enriched sludge and their relative abundances accounted for 9.49 and 4.08%, respectively. Furthermore, it was found that potential FeOB were also abundantly present in activated sludge samples of common municipal wastewater treatment plants. Collectively, this study demonstrated that NDFO could be successfully performed by enriched activated sludge and high diversity of bacteria is involved in this process, and the results also provide baseline information for future research and engineering application of NDFO process.
KeywordsNDFO Microbial community Fe(II)-oxidizing bacteria Activated sludge
This study was funded by the National Natural Science Foundation of China (51608256), the Natural Science Foundation of Jiangsu Province (BK20160657), the Science Foundation for Distinguished Young Scholars of Jiangsu Province of China (BK20150019), and the Fundamental Research Funds for the China Central Universities (021114380030/021114380034).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
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