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Genome-guided exploration of metabolic features of Streptomyces peucetius ATCC 27952: past, current, and prospect

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Abstract

Streptomyces peucetius ATCC 27952 produces two major anthracyclines, doxorubicin (DXR) and daunorubicin (DNR), which are potent chemotherapeutic agents for the treatment of several cancers. In order to gain detailed insight on genetics and biochemistry of the strain, the complete genome was determined and analyzed. The result showed that its complete sequence contains 7187 protein coding genes in a total of 8,023,114 bp, whereas 87% of the genome contributed to the protein coding region. The genomic sequence included 18 rRNA, 66 tRNAs, and 3 non-coding RNAs. In silico studies predicted ~ 68 biosynthetic gene clusters (BCGs) encoding diverse classes of secondary metabolites, including non-ribosomal polyketide synthase (NRPS), polyketide synthase (PKS I, II, and III), terpenes, and others. Detailed analysis of the genome sequence revealed versatile biocatalytic enzymes such as cytochrome P450 (CYP), electron transfer systems (ETS) genes, methyltransferase (MT), glycosyltransferase (GT). In addition, numerous functional genes (transporter gene, SOD, etc.) and regulatory genes (afsR-sp, metK-sp, etc.) involved in the regulation of secondary metabolites were found. This minireview summarizes the genome-based genome mining (GM) of diverse BCGs and genome exploration (GE) of versatile biocatalytic enzymes, and other enzymes involved in maintenance and regulation of metabolism of S. peucetius. The detailed analysis of genome sequence provides critically important knowledge useful in the bioengineering of the strain or harboring catalytically efficient enzymes for biotechnological applications.

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Acknowledgements

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (NRF-2017R1A2A2A05000939 and NRF-2017R1D1A1B03036273).

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  1. Nguyen Huy Thuan and Dipesh Dhakal contributed equally.

Authors and Affiliations

  1. Center for Molecular Biology, Institute of Research and Development, Duy Tan University, 03 Quang Trung Street, Da Nang City, Vietnam

    Nguyen Huy Thuan

  2. Department of Life Science and Biochemical Engineering, Sun Moon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si, Chungnam, 31460, Republic of Korea

    Dipesh Dhakal, Anaya Raj Pokhrel, Luan Luong Chu, Thi Thuy Van Pham, Anil Shrestha & Jae Kyung Sohng

  3. Department of BT-Convergent Pharmaceutical Engineering, Sun Moon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si, Chungnam, 31460, Republic of Korea

    Jae Kyung Sohng

Authors
  1. Nguyen Huy Thuan
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  2. Dipesh Dhakal
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  3. Anaya Raj Pokhrel
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  4. Luan Luong Chu
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  5. Thi Thuy Van Pham
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  7. Jae Kyung Sohng
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Correspondence to Jae Kyung Sohng.

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Thuan, N.H., Dhakal, D., Pokhrel, A.R. et al. Genome-guided exploration of metabolic features of Streptomyces peucetius ATCC 27952: past, current, and prospect. Appl Microbiol Biotechnol 102, 4355–4370 (2018). https://doi.org/10.1007/s00253-018-8957-x

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  • DOI: https://doi.org/10.1007/s00253-018-8957-x

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