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Applied Microbiology and Biotechnology

, Volume 102, Issue 8, pp 3649–3661 | Cite as

Effect of dilution rate on productivity of continuous bacteriophage production in cellstat

  • Dominik Nabergoj
  • Nina Kuzmić
  • Benjamin Drakslar
  • Aleš Podgornik
Biotechnological products and process engineering

Abstract

Ability to efficiently propagate high quantities of bacteriophages (phages) is of great importance considering higher phage production needs in the future. Continuous production of phages could represent an interesting option. In our study, we tried to elucidate the effect of dilution rate on productivity of continuous production of phages in cellstat. As a model system, a well-studied phage T4 and Escherichia coli K-12 as a host were used. Experiments where physiology of bacteria was changing with dilution rate of cellstat and where bacterial physiology was kept constant were performed. For both setups there exists an optimal dilution rate when maximal productivity is achieved. Experimentally obtained values of phage concentration and corresponding productivity were compared with mathematical model predictions, and good agreement was obtained for both types of experiments. Analysis of mathematical model coefficients revealed that latent period and burst size to dilution rate coefficient mostly affect optimum dilution rate and productivity. Due to high sensitivity, it is important to evaluate phage growth parameters carefully, to run cellstat under optimal productivity.

Keywords

Phage T4 E. coli K-12 Cellstat Continuous production Productivity 

Notes

Funding information

This study was funded by Slovenian Research Agency—ARRS (research program no. P3-0387, project no. L4-5532, and project no. 3030-37543). The study was also supported by the European Regional Development Fund and Slovenian Ministry of Education, Science and Sport (project BioPharm.Si).

Compliance with ethical standards

This article does not contain any studies with human participants or animals performed by any of the authors.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Dominik Nabergoj
    • 1
  • Nina Kuzmić
    • 2
  • Benjamin Drakslar
    • 2
  • Aleš Podgornik
    • 1
    • 2
  1. 1.Center of Excellence for Biosensors, Instrumentation and Process Control (COBIK)AjdovščinaSlovenia
  2. 2.Faculty of Chemistry and Chemical TechnologyUniversity of LjubljanaLjubljanaSlovenia

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