Biolubricant potential of exopolysaccharides from the cyanobacterium Cyanothece epiphytica
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Exopolysaccaharides (EPS) are carbohydrate polymers secreted by microbial cells, as a protective layer termed sheath or capsule. Their composition is variable. Optimisation of nutrient factors and the effect of some simple stresses on the ability of Cyanothece epiphytica to produce EPS were tested. Of the tested stresses, exposure to ozone for 50 s at 0.06 mg/L resulted in a relatively high EPS yield, without any damage to cell structure. EPS was characterised physicochemically. Chemically, it was found to be composed of pentoses arabinose and xylose; hexoses glucose, galactose and mannose; and the deoxyhexose fucose sugars which were sulphated and with different functional groups. EPS from C. epiphytica was found to be a good hydrophobic dispersant, an excellent emulsifier as well as a flocculant. Its potential as a biolubricant with characteristics better than the conventional lubricant ‘grease’ was revealed through analysis. This study gave the clue for developing a commercial technology to produce a less expensive and more environment-friendly natural lubricant from the cyanobacterium C. epiphytica for tribological applications.
KeywordsCyanobacteria Extracellular polysaccharide Optimisation Stress Ozone (O3) Biolubricant
The authors acknowledge the Department of Science and Technology-Promotion of University Research and Scientific Excellence (DST-PURSE) program ([SR/FT/LS-113/2009), Centre for Cellular and Molecular Platforms (C-CAMP), Bangalore, India, and the Central Salt and Marine Chemicals Research Institute (CSIR), Bhavnagar, Gujarat, India.
This study was funded by Department of Biotechnology (DBT), government of India for the project on National Repository for Microalgae and Cyanobacteria—Freshwater (BT/PR7005/PBD26/357). The corresponding author (Dr. NT) extends the appreciation to the International Scientific Partnership Program (ISPP), King Saud University for funding the research work through ISPP# 0081.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants or animals performed by any of the authors.
- Coral LA, Zamyadi A, Barbeau B, Bassetti FJ, Lapolli FR, Pre’vost M (2013) Oxidation of Microcystis aeruginosa and Anabaena flos-aquae by ozone: impacts on cell integrity and chlorination by-product formation. Water Res 47:2983–2994. https://doi.org/10.1016/j.watres.2013.03.012 CrossRefPubMedGoogle Scholar
- De Philippis R, Vincenzini M (1998) Exocellular polysaccharides from cyanobacteria and their possible applications. FEMS Microbiol Rev 22:151–175. https://doi.org/10.1111/j.1574-6976.1998.tb00365.x CrossRefGoogle Scholar
- Jacobson BO (1991) Rheology and elastodynamic lubrication. Elsevier, AmsterdamGoogle Scholar
- Jittawuttipoka T, Planchon M, Spalla O, Benzerara K, Guyot F, Cassier-Chauvat C, Chauvat F (2013) Multidisciplinary evidences that Synechocystis PCC6803 exopolysaccharides operate in cell sedimentation and protection against salt and metal stresses. PLoS One 8:e55564. https://doi.org/10.1371/journal.pone.0055564 CrossRefPubMedPubMedCentralGoogle Scholar
- Kielak AM, Castellane TCL, Campanharo JC, Colnago LA, Costa OYA, Corradi da Silva ML, van Veen JA, Lemos EGM, Kuramae EE (2017) Characterization of novel Acidobacteria exopolysaccharides with potential industrial and ecological applications. Sci Rep 7:41193. https://doi.org/10.1038/srep41193 CrossRefPubMedPubMedCentralGoogle Scholar
- Lugt PM (2013) Grease lubrication in rolling bearings. John Wiley & Sons: (Chapter 5). https://doi.org/10.1002/9781118483961.fmatter
- Mota R, Guimaraes R, Buttel Z, Rossi F, Colica G, Silva CJ, Santos C, Gales L, Zille A, De Philippis R, Pereira SB, Tamagnini P (2013) Production and characterization of extracellular carbohydrate polymer from Cyanothece sp. CCY 0110. Carbohydr Polym 92:1408–1415. https://doi.org/10.1016/j.carbpol.2012.10.070 CrossRefPubMedGoogle Scholar
- Pereira S, Zille A, Micheletti E, Moradas-Ferreira P, De Philippis R, Tamagnini P (2009) Complexity of cyanobacterial exopolysaccharides: composition, structures, inducing factors and putative genes involved in their biosynthesis and assembly. FEMS Microbiol Rev 33:917–941. https://doi.org/10.1111/j.1574-6976.2009.00183.x CrossRefPubMedGoogle Scholar
- Spicer SS, Horn RG, Leppi, TJ (1967) Histochemistry of connective tissue mucopolysaccharides. In: Wagner BM (ed) The Connective Tissue, Baltimore, pp 251–303Google Scholar
- Thajuddin N, Subramanian G (2005) Cyanobacterial biodiversity and potential applications in biotechnology. Curr Sci 89:47–57Google Scholar
- Yu HY, Huang J, Chang PR (2015) Surface grafting of cellulose nanowhisker. In: Thakur VK (ed) Cellulose-Based Graft Copolymers. Structure and Chemistry, New York, pp 497–518Google Scholar