Biohydrogen production from fermentation of cotton stalk hydrolysate by Klebsiella sp. WL1316 newly isolated from wild carp (Cyprinus carpio L.) of the Tarim River basin
- 95 Downloads
A new hydrogen-producing bacterium was isolated from the intestine of wild carp (Cyprinus carpio L.) of the Tarim River Basin. The isolate was identified as Klebsiella sp. based on 16S rDNA gene sequencing and examination of physiological and biochemical characteristics. The isolated strain, Klebsiella sp. WL1316, could effectively produce a high yield of hydrogen by using cotton stalk hydrolysate as substrate. The optimum fermentation conditions for hydrogen production were determined as follows: an initial sugar concentration of 40 g/L, a fermentation temperature of 37 °C and an initial pH value of 8.0. The scaled-up fermentation process was conducted in a 5-L fermenter using these parameters. Higher productivities with maximum daily hydrogen production of 937.0 ± 41.0 mL L−1 day−1, cumulative hydrogen production of 2908.5 ± 47.4 mL L−1, viable cell count of (20.2 ± 0.6) × 108 CFU mL−1 and hydrogen yield of 1.44 ± 0.08 mol mol−1sugarconsumed were obtained. The cumulative hydrogen production was predicted by the modified Gompertz equation with R 2 of 0.997, and values of R m and P were 44.8 mL L−1 h−1 and 3057.6 mL L−1, respectively. These results indicated that the strain Klebsiella sp. WL1316 resulted in a high hydrogen production rate (HPR) and good hydrogen production potential. Moreover, this strain exhibited higher values of maximum hydrogen yield and HPR than the reported pure cultures.
KeywordsBiohydrogen production Cotton stalk hydrolysate Klebsiella sp. WL1316 Wild carp Fermentation
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
The protocol of the present study was given approval by the Tarim University Institutional Animal Care and Use Committee. And the procedures of harvest samples followed the Guidelines on Ethical Treatment of Experimental Animals enacted by the Ministry of Science and Technology, China.
- Arreola-Vargas J, Celis LB, Buitrón G, Razo-Flores E, Alatriste-Mondragón F (2013) Hydrogen production from acid and enzymatic oat straw hydrolysates in an anaerobic sequencing batch reactor: performance and microbial population analysis. Int J Hydrog Energy 38:13884–13894. https://doi.org/10.1016/j.ijhydene.2013.08.065 CrossRefGoogle Scholar
- Chookaew T, O-Thong S, Prasertsan P (2012) Fermentative production of hydrogen and soluble metabolites from crude glycerol of biodiesel plant by the newly isolated thermotolerant Klebsiella pneumoniae TR17. Int J Hydrog Energy 37:13314–13322. https://doi.org/10.1016/j.ijhydene.2012.06.022 CrossRefGoogle Scholar
- Chookaew T, O-Thong S, Prasertsan P (2014) Biohydrogen production from crude glycerol by immobilized Klebsiella sp. TR17 in a UASB reactor and bacterial quantification under non-sterile conditions. Int J Hydrog Energy 39:9580–9587. https://doi.org/10.1016/j.ijhydene.2014.04.083 CrossRefGoogle Scholar
- Doetsch RN (1981) Determinative methods of light microscopy. In: Gerhardt P, Murray RGE, Costilow RN, Nester EW, Wood WA, Krieg NR (eds) Manual of methods for general bacteriology. American Society for Microbiology, Washington DC, pp 21–23Google Scholar
- Dong XZ, Cai MY (2001) Identification manual of systematic bacteriology. Science press, Beijing, pp 267–294Google Scholar
- Maintinguer SI, Fernandes BS, Duarte ICS, Saavedra NK, Adorno MAT, Varesche MBA (2011) Fermentative hydrogen production with xylose by Clostridium and Klebsiella species in anaerobic batch reactors. Int J Hydrog Energy 36:13508–13517. https://doi.org/10.1016/j.ijhydene.2011.07.095 CrossRefGoogle Scholar
- Valdez-Vazquez I, Ríos-Leal E, Esparza-García F, Cecchi F, Poggi-Varaldo HM (2005) Semi-continuous solid substrate anaerobic reactors for H2 production from organic waste: mesophilic versus thermophilic regime. Int J Hydrog Energy 30:1383–1391. https://doi.org/10.1016/j.ijhydene.2004.09.016 CrossRefGoogle Scholar
- Wu KJ, Saratale GD, Lo YC, Chen WM, Tseng ZJ, Chang MC, Tsai BC, Su A, Chang JS (2008) Simultaneous production of 2,3-butanediol, ethanol and hydrogen with a Klebsiella sp. strain isolated from sewage sludge. Bioresour Technol 99:7966–7970. https://doi.org/10.1016/j.biortech.2008.03.062 CrossRefPubMedGoogle Scholar
- Wu XB, Huang GF, Bai LP, Long MN, Chen QX (2014) Enhanced hydrogen production from xylose and bamboo stalk hydrolysate by overexpression of xylulokinase and xylose isomerase in Klebsiella oxytoca HP1. Int J Hydrog Energy 39:221–330. https://doi.org/10.1016/j.ijhydene.2013.10.078 CrossRefGoogle Scholar
- Zhang Q, Li Y, Li J, Ma C (2011b) Dilute acid hydrolysis of cotton stalks and ethanol production from hydrolytic liquids. Proceedings 2011 International Conference on Materials for Renewable Energy & Environment (ICMREE 2011) 1: 459–463. https://doi.org/10.1109/ICMREE.2011.5930852