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Applied Microbiology and Biotechnology

, Volume 102, Issue 10, pp 4381–4392 | Cite as

Defining microbial community composition and seasonal variation in a sewage treatment plant in India using a down-flow hanging sponge reactor

  • Naoki Nomoto
  • Masashi Hatamoto
  • Yuga Hirakata
  • Muntjeer Ali
  • Komal Jayaswal
  • Akinori Iguchi
  • Tsutomu Okubo
  • Masanobu Takahashi
  • Kengo Kubota
  • Tadashi Tagawa
  • Shigeki Uemura
  • Takashi Yamaguchi
  • Hideki Harada
Environmental biotechnology

Abstract

The characteristics of the microbial community in a practical-scale down-flow hanging sponge (DHS) reactor, high in organic matter and sulfate ion concentration, and the seasonal variation of the microbial community composition were investigated. Microorganisms related to sulfur oxidation and reduction (2–27%), as well as Leucobacter (7.50%), were abundant in the reactor. Anaerobic bacteria (27–38% in the first layer) were also in abundance and were found to contribute to the removal of organic matter from the sewage in the reactor. By comparing the Simpson index, the abundance-based coverage estimator (ACE) index, and the species composition of the microbial community across seasons (summer/dry, summer/rainy, autumn/dry, and winter/dry), the microbial community was found to change in composition only during the winter season. In addition to the estimation of seasonal variation, the difference in the microbial community composition along the axes of the DHS reactor was investigated for the first time. Although the abundance of each bacterial species differed along both axes of the reactor, the change of the community composition in the reactor was found to be greater along the vertical axis than the horizontal axis of the DHS reactor.

Keywords

DHS (down-flow hanging sponge) Microbial community composition Sewage treatment Developing country 

Notes

Acknowledgments

We are grateful to the National River Conservation Directorate (NRCD); Ministry of Environment, Forest and Climate Change (MOEF); the Indian government; and Uttar Pradesh Jal Nigam (UPJN) for extending various forms of assistance. We also thank Mr. A. Noori and Mr. P. Sjati for contributing to the experiments in India.

Funding

This study was funded by the Science and Technology Research Partnership for Sustainable Development (SATREPS) in Japan.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with animals performed by any of the authors.

Supplementary material

253_2018_8864_MOESM1_ESM.pdf (289 kb)
ESM 1 (PDF 289 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Naoki Nomoto
    • 1
    • 2
  • Masashi Hatamoto
    • 3
  • Yuga Hirakata
    • 4
  • Muntjeer Ali
    • 5
  • Komal Jayaswal
    • 6
  • Akinori Iguchi
    • 7
  • Tsutomu Okubo
    • 8
  • Masanobu Takahashi
    • 5
  • Kengo Kubota
    • 9
  • Tadashi Tagawa
    • 10
  • Shigeki Uemura
    • 8
  • Takashi Yamaguchi
    • 4
  • Hideki Harada
    • 5
  1. 1.Department of Energy and Environment ScienceNagaoka University of TechnologyNiigataJapan
  2. 2.Department of Chemical and Biological EngineeringNational Institute of Technology, Ube CollegeYamaguchiJapan
  3. 3.Department of Civil and Environmental EngineeringNagaoka University of TechnologyNiigataJapan
  4. 4.Department of Science of Technology InnovationNagaoka University of TechnologyNiigataJapan
  5. 5.New Industry Creation Hatchery CenterTohoku UniversitySendaiJapan
  6. 6.Department of Civil EngineeringIndian Institute of Technology RoorkeeRoorkeeIndia
  7. 7.Faculty of Applied Life SciencesNiigata University of Pharmacy and Applied Life SciencesNiigataJapan
  8. 8.Department of Civil EngineeringNational Institute of Technology, Kisarazu CollegeKisarazuJapan
  9. 9.Department of Civil and Environmental EngineeringTohoku UniversitySendaiJapan
  10. 10.Department of Civil EngineeringNational Institute of Technology, Kagawa CollegeTakamatsuJapan

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