Defining microbial community composition and seasonal variation in a sewage treatment plant in India using a down-flow hanging sponge reactor
The characteristics of the microbial community in a practical-scale down-flow hanging sponge (DHS) reactor, high in organic matter and sulfate ion concentration, and the seasonal variation of the microbial community composition were investigated. Microorganisms related to sulfur oxidation and reduction (2–27%), as well as Leucobacter (7.50%), were abundant in the reactor. Anaerobic bacteria (27–38% in the first layer) were also in abundance and were found to contribute to the removal of organic matter from the sewage in the reactor. By comparing the Simpson index, the abundance-based coverage estimator (ACE) index, and the species composition of the microbial community across seasons (summer/dry, summer/rainy, autumn/dry, and winter/dry), the microbial community was found to change in composition only during the winter season. In addition to the estimation of seasonal variation, the difference in the microbial community composition along the axes of the DHS reactor was investigated for the first time. Although the abundance of each bacterial species differed along both axes of the reactor, the change of the community composition in the reactor was found to be greater along the vertical axis than the horizontal axis of the DHS reactor.
KeywordsDHS (down-flow hanging sponge) Microbial community composition Sewage treatment Developing country
We are grateful to the National River Conservation Directorate (NRCD); Ministry of Environment, Forest and Climate Change (MOEF); the Indian government; and Uttar Pradesh Jal Nigam (UPJN) for extending various forms of assistance. We also thank Mr. A. Noori and Mr. P. Sjati for contributing to the experiments in India.
This study was funded by the Science and Technology Research Partnership for Sustainable Development (SATREPS) in Japan.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with animals performed by any of the authors.
- Agrawal LK, Okui H, Ueki Y, Harada H, Ohashi A (1997) Treatment of raw sewage in a temperate climate using a UASB reactor and the hanging sponge cubes process. Water Sci Technol 36:433–440Google Scholar
- APHA (2005) Standard methods, 21st ed. Washington DC, pp 4–136–140Google Scholar
- Clarke KR, Warwick RM (2001) Change in marine communities: an approach to statistical analysis and interpretation, 2nd edn. Natural Environment Research Council, Plymouth Marine Laboratory, PlymouthGoogle Scholar
- Garrity P (2009) Bergey’s manual of systematic bacteriology, vol 3: the Firmicutes. 737. https://doi.org/10.1007/978-0-387-68489-5
- Iguchi A, Okubo T, Tachibana M, Nagai H, Uemura S, Yamaguchi T, Kubota K, Harada H (2013) Bacterial community structure of practical-scale DHS reactor treating municipal wastewater by UASB-DHS system and quantification of denitrifying bacteria. J Jpn Soc Civil Eng Ser G (Environ Res) 69(7):III_539–III_546 (in Japanese)Google Scholar
- Kubota K, Hayashi M, Matsunaga K, Iguchi A, Ohashi A, Li YY, Yamaguchi T, Harada H (2014) Microbial community composition of a down-flow hanging sponge (DHS) reactor combined with an up-flow anaerobic sludge blanket (UASB) reactor for the treatment of municipal sewage. Bioresour Technol 151:144–150CrossRefPubMedGoogle Scholar
- Kuhl M, Jorgensen BB (1992) Microsensor measurements of sulfate reduction and sulfide oxidation in compact microbial communities of aerobic biofilms. Am Soc Microbiol 58(4):1164–1174Google Scholar
- Levén L, Eriksson ARB, Schnürer A (2006) Effect of process temperature on bacterial and archaeal communities in two methanogenic bioreactors treating organic household waste. Fed Eur Microbiol Soc 59:683–693Google Scholar
- Liu L, Liu M, Wilkinson DM, Chen H, Yu X, Yang J (2017) DNA metabarcoding reveals that 200-μm-size-fractionated filtering is unable to discriminate between planktonic microbial and large eukaryotes. Mol Ecol ResourGoogle Scholar
- Machdar I, Sekiguchi Y, Sumino H, Ohashi A, Harada H (2000) Combination of a UASB reactor and a curtain type DHS (downflow hanging sponge) reactor as a cost-effective sewage treatment system for developing countries. Water Sci Technol 42:83–88Google Scholar
- Nomoto N, Ali M, Jayaswal K, Iguchi A, Hatamoto M, Okubo T, Takahashi M, Kubota K, Tagawa T, Uemura S, Yamaguchi T, Harada H (2017a) Characteristics of DO, organic matter, and ammonium profile for practical-scale DHS reactor under various organic load and temperature conditions. Environ Technol, in pressGoogle Scholar
- Nomoto N, Ali M, Jayaswal K, Iguchi A, Hatamoto M, Okubo T, Takahashi M, Kubota K, Tagawa T, Uemura S, Yamaguchi T, Harada H (2017b) Removal and oxygen consumption of retained sludge for organic matter, ammonium, and sulfur in a practical-scale down-flow hanging sponge sewage treatment reactor. In Proceedings of 6th annual international conference on sustainable energy and environmental sciences (SEES 2017), pp 52-58Google Scholar
- Oksanen J, Blanchet FG, Kindt R, Legendre P, Minchin PR, O'Hara RB, Simpson GL, Solymos P, Stevens MHH, Wagner H (2016) Vegan: community ecology package. R package version 2.3–4Google Scholar
- Zheng D, Sun Y, Li H, Lu S, Shan M, Xu S (2016) Multistage A-O activated sludge process for paraformaldehyde wastewater treatment and microbial community structure analysis. J Chem 2016:7Google Scholar