Applied Microbiology and Biotechnology

, Volume 102, Issue 8, pp 3595–3606 | Cite as

The role of magnetic iron oxide nanoparticles in the bacterially induced calcium carbonate precipitation

  • Mostafa Seifan
  • Alireza Ebrahiminezhad
  • Younes Ghasemi
  • Ali Khajeh Samani
  • Aydin Berenjian
Biotechnological products and process engineering


Recently, magnetic iron oxide nanoparticles (IONs) have been used to control and modify the characteristics of concrete and mortar. Concrete is one of the most used materials in the world; however, it is susceptible to cracking. Over recent years, a sustainable biotechnological approach has emerged as an alternative approach to conventional techniques to heal the concrete cracks by the incorporation of bacterial cells and nutrients into the concrete matrix. Once cracking occurs, CaCO3 is induced and the crack is healed. Considering the positive effects of IONs on the concrete properties, the effect of these nanoparticles on bacterial growth and CaCO3 biosynthesis needs to be evaluated for their possible application in bio self-healing concrete. In the present work, IONs were successfully synthesized and characterized using various techniques. The presence of IONs showed a significant effect on both bacterial growth and CaCO3 precipitation. The highest bacterial growth was observed in the presence of 150 μg/mL IONs. The highest concentration of induced CaCO3 (34.54 g/L) was achieved when the bacterial cells were immobilized with 300 μg/mL of IONs. This study provides new data and supports the possibility of using IONs as a new tool in designing the next generation of bio self-healing concrete.


Iron oxide nanoparticle Bacteria CaCO3 Immobilization Bio self-healing concrete Crack 


Funding information

This investigation was financially supported by The University of Waikato, New Zealand.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

This study does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

253_2018_8860_MOESM1_ESM.pdf (180 kb)
ESM 1 (PDF 180 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Mostafa Seifan
    • 1
  • Alireza Ebrahiminezhad
    • 2
    • 3
    • 4
  • Younes Ghasemi
    • 4
  • Ali Khajeh Samani
    • 5
  • Aydin Berenjian
    • 1
  1. 1.School of Engineering, Faculty of Science and EngineeringThe University of WaikatoHamiltonNew Zealand
  2. 2.Noncommunicable Diseases Research CentreFasa University of Medical SciencesFasaIran
  3. 3.Department of Medical Biotechnology, School of MedicineFasa University of Medical SciencesFasaIran
  4. 4.Department of Pharmaceutical Biotechnology, School of Pharmacy, and Pharmaceutical Sciences Research CentreShiraz University of Medical SciencesShirazIran
  5. 5.Faculty of Science and TechnologyFederation University AustraliaBallaratAustralia

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